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A simple and reliable method for determination of optimum pH in coupled enzyme assays

Lee Bowman¹, Rachael Motamed¹, Paul Lee²

¹Department of Chemistry & Physics, Birmingham-Southern College, Birmingham, AL 35254, USA.

|February 15, 2020

Abstract:

Determination of the optimum pH in a coupled enzyme assay poses significant challenges because altering the pH of the reaction mixture can affect the performance of both enzymes. Here, we demonstrate a simple and reliable method to determine the pH optimum for pyruvate kinase using the pyruvate kinase/lactate dehydrogenase coupled enzyme assay. This simple and reliable method can be broadly adapted to determine the pH optimum for various enzymes that are assayed using a coupled enzyme assay.

Keywords:

Cryptosporidium parvum Plasmodium falciparum coupled enzyme assay enzyme kinetics lactate dehydrogenase pH optimization pyruvate kinase

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