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Updated: Dec 28, 2025
Topical Application Bioassay to Quantify Insecticide Toxicity for Mosquitoes and Fruit Flies
Published on: January 19, 2022
Vip3Aa from Bacillus thuringiensis subsp. kurstaki HD1 is toxic to Aedes aegypti (Diptera: Culicidae)
Junhui Wang1, Jiayu Wang1, Jiali He1
1Ministry of Education Key Laboratory for Ecology of Tropical Islands, College of Life Sciences, Hainan Normal University, Haikou 571158, China.
Abstract:
Bacillus thuringensis (Bt) vegetative insecticidal proteins share no sequence or structural homology with the known Cry proteins. The activity of Vip3Aa is generally considered to be restricted to lepidopteran insects. In this paper, the vip3Aa gene was cloned from Bt subsp. kurstaki HD1 and expressed in E. coli. It was found to be toxic to Aedes aegypti larvae - the first time that dipteran activity has been reported. Furthermore, the potential synergism of Vip3Aa with the Cry toxins was investigated. Although no significant synergism was observed, Vip3Aa could be used in combination with Cry toxins to prevent or delay the onset of resistance.
