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Related Experiment Video

Updated: Dec 28, 2025

Gramicidin-based Fluorescence Assay; for Determining Small Molecules Potential for Modifying Lipid Bilayer Properties
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Moxifloxacin interacts with lipid bilayer, causing dramatic changes in its structure and phase transitions.

Irina M Le-Deygen1, Anna A Skuredina1, Anastasia S Safronova1

  • 1Lomonosov MSU, Faculty of Chemistry, Chemical Enzymology Department, 119991, Moscow, Leninskie Gory, 1, 3, Russia.

Chemistry and Physics of Lipids
|February 15, 2020
PubMed
Summary

Moxifloxacin (Mox) interaction with lipid bilayers depends on liposome composition. Anionic liposomes stabilize the membrane, while neutral liposomes lead to defects and rapid drug release.

Keywords:
Biochemical analysisDSCFTIRFluorescenceFluoroquinolonesLiposomesMoxifloxacinSpectroscopy

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Area of Science:

  • Biophysics
  • Pharmacology
  • Materials Science

Background:

  • Nonsteroidal anti-inflammatory drugs can cause undesired side effects by interacting with cell membranes.
  • Understanding drug-membrane interactions is crucial for predicting drug behavior and side effects.

Purpose of the Study:

  • To investigate the mechanism of moxifloxacin (Mox) interaction with lipid bilayers as a cell membrane model.
  • To determine how liposome composition influences Mox binding, localization, and effects on membrane properties.

Main Methods:

  • Attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy
  • Circular dichroism (CD) spectroscopy
  • UV-Vis spectroscopy
  • Fluorescence spectroscopy
  • Differential scanning calorimetry (DSC)

Main Results:

  • Mox interaction strength and character are significantly affected by lipid composition.
  • In anionic liposomes (DPPC/CL2-), electrostatic interactions promote stable complex formation, stabilizing the gel state and increasing phase transition temperature (Tm) by 3-5 °C.
  • In neutral liposomes (DPPC), Mox causes defects, fluidizes the bilayer, and decreases Tm by 2-4 °C, leading to rapid drug release.

Conclusions:

  • Lipid composition dictates moxifloxacin's interaction with cell membrane models.
  • Anionic lipids enhance Mox binding and membrane stability, whereas neutral lipids promote drug release.