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Multiple Progressive Thermopreconditioning Improves Cardiac Ischemia/Reperfusion-induced Left Ventricular Contractile
Yueh-Hsi Chen1, Chih-Yao Chiang1,2,3, Tzu-Ching Chang1
1School of Life Science, National Taiwan Normal University, Taipei, Taiwan, Republic of China.
Transplantation
|February 15, 2020
Summary
Triple progressive thermopreconditioning (3PTP) protects the heart from ischemia/reperfusion injury by upregulating Bag3 and Hsp-70. This method may reduce organ transplant damage through these protective mechanisms.
Area of Science:
- Cardiovascular Science
- Transplantation Medicine
- Cellular Stress Response
Background:
- Triple progressive thermopreconditioning (3PTP) may enhance heat shock protein 70 (Hsp-70) expression, crucial for maintaining cardiac function.
- The study investigates 3PTP's potential to mitigate myocardial ischemia/reperfusion (I/R) injury during organ transplantation.
- A proposed mechanism involves Bag3 and Hsp-70 mediated defense pathways.
Purpose of the Study:
- To evaluate the cardioprotective effects of 3PTP against ischemia/reperfusion (I/R) injury in a rat model.
- To elucidate the molecular mechanisms underlying 3PTP-induced cardioprotection, focusing on Bag3 and Hsp-70.
- To assess the impact of 3PTP on cardiac function, microcirculation, and infarct size post-I/R.
Main Methods:
- Male Wistar rats were subjected to 60 minutes of ischemia followed by 240 minutes of reperfusion.
- Groups included a sham control and a 3PTP group (preconditioned 72 hours prior).
- Evaluated hemodynamic parameters, cardiac microcirculation, infarct size, and protein expression (Western blot, immunohistochemistry).
Main Results:
- 3PTP significantly reduced I/R-induced cardiac dysfunction, infarct size, and inflammatory cell infiltration.
- Cardioprotection was associated with increased expression of Hsp-70 and Bag3.
- The Bax/Bcl-2 ratio was decreased, indicating reduced apoptosis, while autophagy markers remained unaffected.
Conclusions:
- 3PTP therapy effectively alleviates myocardial I/R injury.
- Bag3 upregulation plays a key role in mitigating left ventricular structural damage and dysfunction caused by I/R.
- 3PTP represents a promising preconditioning strategy for reducing organ transplant-related cardiac injury.
