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Development of polyclonal-antibody-coated immunomagnetic beads for separation and detection of koi herpesvirus in

Min Zhang1, Yingying Li2, Hongli Jing1

  • 1The Institute of Animal Quarantine, Chinese Academy of Inspection and Quarantine, No. 11 Building, Ronghua South Road, Daxing District, Beijing, 100176, China.

Archives of Virology
|February 16, 2020
PubMed

To separate and concentrate koi herpesvirus (KHV) from large-volume samples, a separation method based on immunomagnetic beads (IMBs) coated with polyclonal antibody directed against KHV was developed. After treatment with IMBs, viral DNA was extracted from samples and used as a template for quantitative PCR (qPCR). The results showed that the concentration of the template DNA extracted from the virus that had been separated using IMBs was 9.65-fold higher than that from virus not treated with IMBs. The detection limit of the IMBs/qPCR method was found to be at least 10 times lower than that of qPCR alone.

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