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In vivo Spectroscopic Evaluation of the Intraperitoneal Cavity in Canines
Gwendolyn M Cramer1, Julia Sandell Meo2, Jarod C Finlay1
1Department of Radiation Oncology, University of Pennsylvania, Philadelphia, PA, United States.
Photochemistry and Photobiology
|February 16, 2020
Summary
Photodynamic therapy (PDT) using benzoporphyrin derivative (BPD) shows promise for peritoneal carcinomatosis. Tissue properties varied significantly, highlighting the need for personalized PDT dosimetry for effective treatment.
Area of Science:
- Biomedical Optics
- Photodynamic Therapy
- Surgical Oncology
Background:
- Peritoneal carcinomatosis (PC) presents a challenge for superficial disease treatment.
- Photodynamic therapy (PDT) efficacy depends on tissue optical properties and oxygenation.
- Benzoporphyrin derivative (BPD)-mediated PDT is a potential treatment for PC.
Purpose of the Study:
- To assess changes in optical properties, tissue oxygenation, and drug concentration during BPD-mediated PDT.
- To evaluate the influence of tissue heterogeneity on PDT treatment efficacy.
- To investigate BPD photobleaching during PDT.
Main Methods:
- Preclinical study in healthy canines undergoing partial bowel resection.
- In-vivo spectroscopic and fluorescence measurements using a custom probe.
- Diffuse reflectance spectroscopy to determine optical properties, hemoglobin concentration, and oxygenation.
Main Results:
- Significant heterogeneity in hemoglobin concentration, oxygenation, and optical properties observed between subjects and anatomical locations.
- No significant BPD photobleaching was detected post-PDT.
- Spectroscopic measurements provided insights into tissue response to PDT.
Conclusions:
- Patient and location-specific dose optimization is crucial for developing effective PDT dosimetry systems.
- BPD does not appear to photobleach, suggesting sustained photosensitizing potential.
- Understanding tissue heterogeneity is key to advancing BPD-mediated PDT for PC.
