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Related Experiment Video

Updated: Dec 28, 2025

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
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Measuring diffusion exchange across the cell membrane with DEXSY (Diffusion Exchange Spectroscopy).

James O Breen-Norris1,2, Bernard Siow1,2,3, Claire Walsh1

  • 1Centre for Advanced Biomedical Imaging, Division of Medicine, University College London, London, UK.

Magnetic Resonance in Medicine
|February 16, 2020
PubMed
Summary
This summary is machine-generated.

Diffusion exchange spectroscopy (DEXSY) shows promise for measuring cell membrane permeability. This technique was validated through simulations, in vitro, and in vivo experiments, demonstrating its feasibility for in vivo diffusion exchange measurements.

Keywords:
DEXSYDiffusion exchangeFEXSYcell membrane permeability

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Area of Science:

  • Biophysics
  • Cell Biology
  • Medical Imaging

Background:

  • Cell membrane permeability is crucial for cellular function and drug delivery.
  • Accurate measurement of diffusion exchange across membranes is challenging.
  • Diffusion Exchange Spectroscopy (DEXSY) is an emerging technique for studying molecular motion.

Purpose of the Study:

  • To evaluate the feasibility of using DEXSY to measure diffusion exchange across cell membranes.
  • To validate DEXSY measurements through numerical simulations and experimental approaches.
  • To assess DEXSY's potential for in vivo applications.

Main Methods:

  • Numerical simulations of DEXSY acquisitions across a range of permeabilities.
  • In vitro experiments using yeast substrates at 9.4 T.
  • In vivo experiments utilizing mouse tumor xenograft models at 9.4 T.

Main Results:

  • Simulations demonstrated diffusion exchange over physiologically relevant permeability values.
  • In vitro and in vivo experiments provided evidence of diffusion exchange.
  • The Diffusion Exchange Index (DEI) was developed to quantify diffusion exchange.

Conclusions:

  • DEXSY shows potential for in vivo measurement of diffusion exchange.
  • The study provides preliminary evidence for DEXSY's utility in assessing cell membrane permeability.
  • DEXSY offers a novel approach for non-invasive characterization of membrane transport.