Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Sampling Methods: Overview

Sampling Methods: Overview

A sample refers to a smaller subset representative of a larger population. In analytical chemistry, studying or analyzing an entire population is often impractical or impossible. Therefore, samples are used to draw inferences and generalize the whole population. The sampling method selects individuals or items from a population to create a sample. Standard sampling methods include random, judgemental, systematic, stratified, and cluster sampling.
In analytical chemistry, the choice of...

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

Rapidly Varying Flow

Rapidly Varying Flow

Rapidly varying flow (RVF) in open channels is characterized by abrupt changes in flow depth over a short distance, with the rate of depth change relative to distance often approaching unity. These flows are inherently complex due to their transient and multi-dimensional nature, making exact analysis difficult. However, approximate solutions using simplified models provide valuable insights into their behavior.Key Features of Rapidly Varying FlowRVF is commonly observed in scenarios involving...

Sampling Plans

Sampling Plans

Sampling is a crucial step in analytical chemistry, allowing researchers to collect representative data from a large population. Common sampling methods include random, judgmental, systematic, stratified, and cluster sampling.
Random sampling is a method where each member of the population has an equal chance of being selected for the sample. It involves selecting individuals randomly, often using random number generators or lottery-type methods. For example, when analyzing the properties of a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

How to quantify and avoid finite size effects in computational studies of crystal nucleation: The case of heterogeneous ice nucleation.

The Journal of chemical physics·2021

Same author

Forward-flux sampling with jumpy order parameters.

The Journal of chemical physics·2018

Same author

Comment on "The putative liquid-liquid transition is a liquid-solid transition in atomistic models of water" [I and II: J. Chem. Phys. 135, 134503 (2011); J. Chem. Phys. 138, 214504 (2013)].

The Journal of chemical physics·2018

Same author

Forward flux sampling calculation of homogeneous nucleation rates from aqueous NaCl solutions.

The Journal of chemical physics·2018

Search research articles

Related Experiment Video

Updated: Dec 28, 2025

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

Published on: October 9, 2014

Studying rare events using forward-flux sampling: Recent breakthroughs and future outlook.

Sarwar Hussain¹, Amir Haji-Akbari¹

¹Department of Chemical and Environmental Engineering, Yale University, New Haven, Connecticut 06520, USA.

The Journal of Chemical Physics

|February 17, 2020

Summary

Rare events, crucial for understanding structural changes in biology and materials, are challenging to study. Forward flux sampling is a key computational technique for analyzing these improbable fluctuations and their mechanisms.

More Related Videos

Quantifying Spatiotemporal Parameters of Cellular Exocytosis in Micropatterned Cells

Quantifying Spatiotemporal Parameters of Cellular Exocytosis in Micropatterned Cells

Published on: September 16, 2020

Lensless Fluorescent Microscopy on a Chip

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

Related Experiment Videos

Last Updated: Dec 28, 2025

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

Published on: October 9, 2014

Quantifying Spatiotemporal Parameters of Cellular Exocytosis in Micropatterned Cells

Quantifying Spatiotemporal Parameters of Cellular Exocytosis in Micropatterned Cells

Published on: September 16, 2020

Lensless Fluorescent Microscopy on a Chip

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

Area of Science:

Computational Chemistry
Statistical Mechanics
Biophysics

Background:

Rare events, driven by unlikely fluctuations, are fundamental to many natural processes, including structural transformations in biology and material science.
Studying the kinetics and molecular mechanisms of these barrier crossings is essential for predicting and controlling system properties.
Conventional experimental and computational methods struggle to capture these rare events due to their activated nature.

Purpose of the Study:

To provide a comprehensive overview of the forward flux sampling (FFS) technique for studying rare events.
To highlight recent methodological advancements and extensions of FFS.
To review the diverse applications of FFS in various scientific domains.

Main Methods:

Focus on the forward flux sampling (FFS) computational technique.
Overview of FFS methodological variants and extensions.
Detailed review of FFS applications for studying rare events.

Main Results:

FFS enables the systematic capture of improbable fluctuations relevant to rare events.
Recent variants and extensions have broadened the applicability and efficiency of FFS.
FFS has been successfully applied to a wide array of rare event phenomena.

Conclusions:

Forward flux sampling is a powerful computational tool for investigating rare events.
Continued development of FFS promises further insights into complex natural phenomena.
This perspective maps future research directions for advanced sampling techniques.