Effects of pH and nitrogen form on Nitzschia closterium growth by linking dynamic with enzyme activity

In this study, Nitzschia closterium was incubated in seawater at different pH values (8.10, 7.71, and 7.45) and using different nitrogen forms (NO₃-N and NH₄-N) in the laboratory. The results showed that the growth of N. closterium was inhibited by ocean acidification, with individuals under lower pH levels showing lower growth rates and lower nitrogen uptake rates for both nitrogen forms. The V_max/K_s ratio decreased with decreasing pH, indicating the inhibition of nitrogen uptake, whereas the ratios for NH₄-N cultures were higher than those for NO₃-N cultures, implying the highly competitive position of NH₄-N. Acidification might induce reactive oxygen species based on the result that the maximum enzyme activities of SuperOxide Dismutase (SOD) and CATalase (CAT) increased under lower pH levels. The SOD and CAT activities for the NO₃-N cultures were higher than those for NH₄-N cultures at the low pH level, indicating that acidification might cause more oxidative stress for NO₃-N cultures than for NH₄-N cultures. Thus, ocean acidification might have a more detrimental effect on the growth of N. closterium under NO₃-N conditions than NH₄-N conditions, with a lower ratio (γ) of the maximum growth rate to the maximum nutrient uptake rate, and a drop in nitrate reductase activity under lower pH levels.