Ohmic heating polyphenolic extracts from vine pruning residue with enhanced biological activity

Area of Science:

• Agricultural Science
• Food Science
• Biotechnology

Background:

• Vine pruning residue is a significant agricultural byproduct.
• Bioactive compounds in plant residues offer potential health benefits.
• Efficient extraction methods are crucial for utilizing these compounds.

Purpose of the Study:

• To extract bioactive compounds from vine pruning residue using conventional and ohmic heating (OH).
• To evaluate the antioxidant, antimicrobial, and anticancer activities of the extracts.
• To compare the efficacy of different OH electric field strengths.

Main Methods:

• Extraction of bioactive compounds using conventional heating and OH (Low and Intermediate electric field - IEF).
• Analysis of total phenolic content (TPC), polyphenolic profile, antioxidant, antimicrobial, and anticancer activities.
• Testing on various tumor cell lines (HepG2, MDA-MB-231, MCF-7, Caco2).

Main Results:

• OH extraction, particularly with IEF, significantly increased TPC compared to conventional heating.
• Higher TPC correlated with enhanced antioxidant, antimicrobial, and anti-proliferative activity.
• The IEF OH extract demonstrated notable anticancer potential against tested cell lines.

Conclusions:

• Ohmic heating is an effective and eco-friendly method for extracting bioactive compounds from vine pruning residue.
• The extracts possess significant antioxidant and anticancer activities.
• Vine pruning residue is a valuable source for developing natural health products.