Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An Evaluation of Cage Amine Sarcophagine Ligands for the Synthesis of Cobalt Radiopharmaceuticals.

Inorganic chemistry·2026
Same author

Discovering Serum Stable Protein-Protein Interaction Inhibitors with N‑Terminus-Capped Bicyclic Phage Libraries.

JACS Au·2026
Same author

Lan enzyme-free construction of lanthionine-bridged macrocyclic phage libraries.

Chemical science·2026
Same author

Preferential Formation and Stability of Copper Porphyrins amidst Other Metals in Dilute Aqueous Solution─Applications for Radiopharmaceuticals.

Inorganic chemistry·2026
Same author

Immunity in Harmony: Utilizing Overlapping Epitopes for Tuberculosis and COVID-19 Protection.

Protein and peptide letters·2026
Same author

Investigating the Theranostic Potential of Elementally Matched [<sup>43</sup>Sc]Sc-PSMA-617 and [<sup>47</sup>Sc]Sc-PSMA-617.

Molecular pharmaceutics·2026

Related Experiment Video

Updated: Dec 28, 2025

In Vivo Imaging of Transduction Efficiencies of Cardiac Targeting Peptide
09:02

In Vivo Imaging of Transduction Efficiencies of Cardiac Targeting Peptide

Published on: June 11, 2020

5.8K

Radiolabeled Cationic Peptides for Targeted Imaging of Infection.

Tolulope A Aweda1, Zumrut F B Muftuler2, Adriana V F Massicano1

  • 1Department of Radiology, University of Alabama at Birmingham, Birmingham, AL, USA.

Contrast Media & Molecular Imaging
|February 18, 2020
PubMed
Summary

Researchers developed novel positron emission tomography (PET) probes, AB1-HLys-DOTA, for imaging bacterial infections. These probes show high uptake in Staphylococcus aureus infections, enabling effective in vivo detection.

More Related Videos

MR Molecular Imaging of Prostate Cancer with a Small Molecular CLT1 Peptide Targeted Contrast Agent
06:54

MR Molecular Imaging of Prostate Cancer with a Small Molecular CLT1 Peptide Targeted Contrast Agent

Published on: September 3, 2013

11.6K
Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT
11:34

Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT

Published on: April 29, 2017

9.2K

Related Experiment Videos

Last Updated: Dec 28, 2025

In Vivo Imaging of Transduction Efficiencies of Cardiac Targeting Peptide
09:02

In Vivo Imaging of Transduction Efficiencies of Cardiac Targeting Peptide

Published on: June 11, 2020

5.8K
MR Molecular Imaging of Prostate Cancer with a Small Molecular CLT1 Peptide Targeted Contrast Agent
06:54

MR Molecular Imaging of Prostate Cancer with a Small Molecular CLT1 Peptide Targeted Contrast Agent

Published on: September 3, 2013

11.6K
Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT
11:34

Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT

Published on: April 29, 2017

9.2K

Area of Science:

  • Molecular imaging
  • Radiochemistry
  • Infectious disease diagnostics

Background:

  • Bacterial infections pose significant health challenges, necessitating advanced diagnostic tools.
  • Molecular probes offer potential for in vivo imaging and therapy of bacterial infections.

Purpose of the Study:

  • To develop and evaluate novel positron emission tomography (PET) probes for detecting bacterial infections in vivo.
  • To assess the efficacy of 64Cu-labeled AB1-HLys-DOTA and 64Cu-HLys-DOTA as PET imaging agents.

Main Methods:

  • Synthesis and radiolabeling of HLys-DOTA and AB1-HLys-DOTA peptides with 64Cu.
  • In vitro cell uptake studies using Staphylococcus aureus (SA).
  • In vivo PET imaging and biodistribution studies in intramuscularly infected mice models.

Main Results:

  • 64Cu-AB1-HLys-DOTA demonstrated significantly higher cell uptake (98.47%) compared to 64Cu-HLys-DOTA (39.12%) in SA at 24 hours.
  • In vivo studies showed 4-fold higher uptake of 64Cu-AB1-HLys-DOTA in infected muscles compared to 64Cu-HLys-DOTA.
  • The probe successfully distinguished between SA-infected and Pseudomonas aeruginosa-infected muscle.

Conclusions:

  • 64Cu-labeled AB1-HLys-DOTA is a promising PET imaging probe for detecting bacterial infections in vivo.
  • The AB1 targeting group enhances probe accumulation in infected tissues, offering specificity for Gram-positive bacteria.