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MiR-769-5p inhibits cancer progression in oral squamous cell carcinoma by directly targeting JAK1/STAT3 pathway

Y Zhou1, X M Xu2, Y Feng3

  • 1Department of Stomatology, Hospital of 363, Chengdu, China.

Neoplasma
|February 18, 2020
PubMed

Insights

MicroRNA-769-5p (miR-769-5p) suppresses oral squamous cell carcinoma (OSCC) development by targeting Janus kinase 1 (JAK1). This finding offers a potential new therapeutic strategy for OSCC patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Oral squamous cell carcinoma (OSCC) is a prevalent and deadly cancer.
  • The role of microRNA-769-5p (miR-769-5p) in OSCC pathogenesis is not fully understood.
  • Previous studies suggest miR-769-5p is downregulated in OSCC.

Purpose of the Study:

  • To investigate the role and mechanism of miR-769-5p in OSCC.
  • To determine if miR-769-5p targets Janus kinase 1 (JAK1) in OSCC.
  • To evaluate the therapeutic potential of miR-769-5p in OSCC.

Main Methods:

  • Quantitative real-time PCR and Western blot to assess miR-769-5p and JAK1 expression.
  • Cellular assays (CCK-8, Transwell, flow cytometry) to evaluate proliferation, migration, invasion, and apoptosis.
  • Dual-luciferase reporter assay to confirm the interaction between miR-769-5p and JAK1.
  • In vivo subcutaneous transplantation experiments in mice.

Main Results:

  • MiR-769-5p was significantly downregulated, while JAK1 was upregulated in OSCC tissues and cells.
  • MiR-769-5p inhibited OSCC cell proliferation, migration, and invasion, while promoting apoptosis.
  • JAK1 was identified as a direct target of miR-769-5p, and its reintroduction reversed the effects of miR-769-5p.
  • MiR-769-5p suppressed OSCC tumor growth in vivo.

Conclusions:

  • MiR-769-5p acts as a tumor suppressor in OSCC by targeting JAK1.
  • The miR-769-5p/JAK1 axis influences apoptosis and epithelial-mesenchymal transition (EMT)-related proteins.
  • Targeting the JAK1/STAT3 pathway via miR-769-5p represents a promising therapeutic strategy for OSCC.

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