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Related Experiment Video

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Quantifying Liver Size in Larval Zebrafish Using Brightfield Microscopy
07:35

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Quantifying Liver Size in Larval Zebrafish Using Brightfield Microscopy.

Srishti Kotiyal1, Alexis Fulbright1, Liam K O'Brien1

  • 1Department of Pathology, Department of Oncological Sciences, and Huntsman Cancer Institute, University of Utah School of Medicine.

Journal of Visualized Experiments : Jove
|February 18, 2020
PubMed
Summary

This study presents a protocol for quantifying larval liver size in zebrafish hepatocellular carcinoma (HCC) models. This method allows rapid assessment of drug effects on liver growth and provides insights into liver development and regeneration.

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Area of Science:

  • Zebrafish models
  • Hepatocellular carcinoma (HCC) research
  • Developmental biology

Background:

  • Hepatomegaly is a common early phenotype in zebrafish models of hepatocellular carcinoma (HCC).
  • Accurate quantification of larval liver size is crucial for assessing oncogene-related phenotypes and the efficacy of therapeutic interventions.
  • Existing methods may not offer the speed and precision required for high-throughput screening.

Purpose of the Study:

  • To establish a rapid and precise protocol for quantifying larval liver size in zebrafish HCC models.
  • To provide a standardized method for assessing drug effects and genetic manipulations on liver growth.
  • To enable detailed visualization of liver, gut, and pancreas for downstream analyses.

Main Methods:

  • Fixation of zebrafish larvae.
  • Dissection to isolate liver tissue while preserving surrounding organs.
  • Bright-field microscopy for imaging.
  • Image analysis to measure liver area.
  • Statistical analysis of results.

Main Results:

  • The developed protocol enables rapid and precise quantification of larval liver size.
  • The method allows for efficient assessment of liver size changes in response to oncogenic stimuli.
  • The dissection technique facilitates visualization of liver, gut, and pancreas in situ.

Conclusions:

  • This protocol offers a valuable tool for studying liver development, regeneration, and disease in zebrafish.
  • The method's efficiency supports its application in drug screening and genetic studies of HCC.
  • Complementary methods are recommended to distinguish between changes in cell size and cell number when assessing liver volume.