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Morphometric analysis of sperm used for IVP by three different separation methods with spatial light interference

Marcello Rubessa1,2, Mikhail E Kandel3, Sierra Schreiber2

  • 1Carl R. Woese Institute for Genomic Biology, University of Illinois, Urbana, IL, USA.

Systems Biology in Reproductive Medicine
|February 19, 2020
PubMed
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Sperm selection methods for bovine in vitro fertilization create distinct sperm subpopulations. Spatial light interference microscopy (SLIM) and computer-assisted semen analysis (CASA) revealed significant differences in sperm morphology and motility.

Area of Science:

  • Reproductive Biology
  • Spermatozoa Analysis
  • In Vitro Fertilization Technologies

Background:

  • Effective sperm selection is crucial for successful bovine in vitro fertilization (IVF).
  • Traditional methods may yield varied sperm subpopulations, impacting IVF outcomes.
  • Novel technologies are needed to accurately characterize selected sperm populations.

Purpose of the Study:

  • To characterize and compare sperm subpopulations resulting from three different sperm selection methods for bovine IVF.
  • To evaluate the effectiveness of discontinuous Percoll gradients, Swim-Up, and electro-channel selection techniques.
  • To investigate the potential of Spatial Light Interference Microscopy (SLIM) and Computer-Assisted Semen Analysis (CASA) in assessing sperm quality.

Main Methods:

Keywords:
CASAIVFSLIMbovinemorphometrysperm

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  • Employed Spatial Light Interference Microscopy (SLIM) for detailed sperm morphology analysis.
  • Utilized Computer-Assisted Semen Analysis (CASA) to assess sperm motility parameters.
  • Analyzed frozen-thawed Holstein bull spermatozoa selected via Percoll gradients, Swim-Up, and electro-channel methods.

    • Main Results:

    • Demonstrated that Percoll gradients, Swim-Up, and electro-channel selection produce significantly different sperm subpopulations.
    • Observed statistically significant differences in almost all evaluated morphometric and kinetic parameters between groups.
    • Novel finding: Spermatozoa selected via electro-channel exhibited a heavier dry mass in the sperm head compared to other methods.

    Conclusions:

    • The choice of sperm selection method critically influences the characteristics of the resulting sperm population for bovine IVF.
    • SLIM microscopy offers valuable insights into sperm morphology, showing significant potential in reproductive biology research.
    • Further research is warranted to optimize selection techniques based on these findings for improved IVF efficiency.