Diffusion Kurtosis Imaging of Leptin Intervention in Early Hypoxic-Ischemic Brain Edema

The role of leptin in neuroprotection has recently been recognized. However, there are few reports on the use of imaging methods to dynamically evaluate the neuroprotection role of leptin. Diffusion kurtosis imaging (DKI), which is a method used to measure non-Gaussian water diffusion, can reflect the real water diffusion in brain tissues. In this study, a newborn piglet model was used to dynamically evaluate the leptin intervention in early hypoxic-ischemic brain edema via DKI. Thirty-two Yorkshire newborn piglets were divided into three groups: the hypoxic-ischemic encephalopathy (HIE) group, the leptin group, and the control group. DKI scanning was performed at time points of 3, 6, 9, 12, 16, and 24 h after hypoxic-ischemic exposure. After scanning, arterial blood was extracted from all piglets to measure NSE and S100β levels. Then, the brain was completely extracted for pathological examination. In the lesion areas, the MK, Ka, and Kr values in the leptin group were significantly lower than those in the HIE group, the MD, Da, and Dr values showed an opposite trend. The lesion areas in the leptin group were smaller than those of in the HIE group. In addition, the pathological results showed that less cell and organelle injury occurred in the leptin group. Our findings indicate that leptin can effectively reduce hypoxic-ischemic brain edema, and DKI can be more sensitive than conventional diffusivity metrics for visualizing the microstructural changes of HIE. This provides a new clue for the treatment and evaluation of HIE.