A Novel Approach for the Quantification of Different Inorganic and Organic Phosphorus Compounds in Environmental...

Phosphorus (P) is an essential element for life on Earth, with an important and oftentimes unaccounted organic biogeochemical component. Current methods for the quantification of different organic P compounds in environmental samples (e.g., soils, sediments) are based on extraction techniques and often associated with incomplete P recovery or sample changes. In this study, we present a protocol for the quantification of different organic and inorganic P species in soils using synchrotron-based X-ray absorption near-edge structure (XANES) spectroscopy at the P L_2,3-edge. Its accuracy and precision was evaluated by analyzing 40 standard mixtures composed of seven different inorganic and organic P compounds (with a mean of R² = 0.85). In addition, we quantified the P species of two soils and two agro-industrial byproducts using P L_2,3-edge XANES spectroscopy and the results were compared with those obtained by P K-edge XANES or ³¹P NMR spectroscopy. Using the P L_2,3-edge, we identified different organic P species, including those not identified by the common P K-edge XANES. However, there is a consistent underestimation of organic polyphosphates. Overall, the application of P L_2,3-edge XANES provides a higher level of information than by P K-edge XANES, although the ubiquitous use of this novel methodology is still limited to samples with a phosphorus content above 3 mg g^-1.