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[Changes in the "latent" virulence of a vaccinal strain of Yersinia pestis multiplying within macrophages]
Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
|September 1, 1988
Abstract:
The multiplication of Y. pestis vaccinal strain inside peritoneal macrophages of guinea pigs and white mice in vitro leads to an essential increase in its latent virulence. This effect is most pronounced when guinea pig macrophages are used. Changes in the latent virulence of Y. pestis vaccinal strains, occurring in the process of their passage inside macrophages in vitro, correlate with those observed in vivo, i.e. in animal experiments.
Insights
Multiplying Yersinia pestis (Y. pestis) vaccinal strains within macrophages increases their virulence. This effect was most significant in guinea pig macrophages, mirroring in vivo findings.
Area of Science:
- Immunology
- Microbiology
- Pathogen-host interactions
Background:
- Yersinia pestis (Y. pestis) is a bacterium responsible for plague.
- Vaccinal strains are developed to elicit protective immunity.
- Understanding pathogen adaptation within host cells is crucial for vaccine efficacy.
Purpose of the Study:
- To investigate the impact of intracellular multiplication within macrophages on the virulence of Y. pestis vaccinal strains.
- To compare the effects of guinea pig and mouse macrophages on Y. pestis virulence.
- To correlate in vitro findings with in vivo observations.
Main Methods:
- Culturing Y. pestis vaccinal strains within peritoneal macrophages isolated from guinea pigs and mice.
- Assessing changes in latent virulence following intracellular passage.
- Comparing in vitro results with established in vivo experimental data.
Main Results:
- Intracellular multiplication of Y. pestis vaccinal strains within macrophages significantly increased latent virulence.
- This virulence enhancement was most pronounced in guinea pig macrophages compared to mouse macrophages.
- Observed in vitro virulence changes correlated with in vivo experimental outcomes.
Conclusions:
- Macrophages can modulate the virulence of Y. pestis vaccinal strains.
- Guinea pig macrophages appear to induce a greater virulence increase than mouse macrophages.
- In vitro macrophage passage serves as a relevant model for predicting in vivo virulence changes of Y. pestis vaccinal strains.