Noble Metal-Assisted Surface Plasmon Resonance Immunosensors

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Biosensing Technology

Background:

• Early disease diagnosis relies on detecting biomarkers in bodily fluids.
• Immunosensors, leveraging antigen-antibody reactions, are key analytical tools.
• Surface plasmon resonance (SPR)-based immunosensors offer high sensitivity and selectivity.

Purpose of the Study:

• To review SPR-based immunosensors for protein biomarker detection.
• To highlight the advantages of SPR immunosensors over traditional methods like ELISA.
• To introduce SPR platforms utilizing noble metals (Au, Ag) for biomarker measurement.

Main Methods:

• Review of literature on SPR-based immunosensors.
• Focus on SPR-inducing factors like gold (Au) and silver (Ag).
• Application in detecting various protein biomarkers, including viruses, microbes, and extracellular vesicles (EVs).

Main Results:

• SPR-based immunosensors enable label-free detection of biomarkers.
• These sensors demonstrate high sensitivity and selectivity for diverse targets.
• Noble metal-based SPR platforms are effective for protein biomarker quantification.

Conclusions:

• SPR-based immunosensors represent a promising platform for sensitive and selective disease biomarker detection.
• Label-free detection capabilities simplify assay procedures compared to methods requiring enzyme labels.
• The use of noble metals enhances the performance of SPR immunosensors for various protein biomarkers.