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Structural characterization and immune enhancement activity of a novel polysaccharide from Moringa oleifera leaves
Chao Li1, Zhuping Dong2, Bin Zhang1
1School of Food Science and Engineering, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, National Joint Research Center for Tropical Health Foods, South China University of Technology, Guangzhou 510640, China; Overseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111 Center), Guangzhou, China.
Abstract:
A novel polysaccharide, designated as MOP-3, was extracted and isolated from the leaves of Moringa oleifera. Structural characterization showed that MOP-3 had a molecular weight (MW) of 4.033 × 106 Da and was composed of arabinose, glucose and galactose with a molar ratio of 47.73:1.00:57.65. Methylation and nuclear magnetic resonance (NMR) analysis revealed that the main glycosidic linkage types of MOP-3 were (1→3,6)-β-D-Galp, (1→6)-β-D-Galp, (1→5)-α-L-Araf, and T-α-L-Araf. MOP-3 containing no lipopolysaccharides showed no direct cytotoxicity to the macrophage RAW 264.7 cells within the tested concentrations. The immunomodulatory assay suggested that MOP-3 could significantly enhance pinocytic capacity and increase the secretion of reactive oxygen species (ROS), nitric oxide (NO), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) by up-regulating the corresponding mRNA expression levels in RAW 264.7 cells. Our findings demonstrated that MOP-3 could potentially be used as a functional food for the hypoimmunity therapies.
