A miniaturized and high sensitive dual channel fluorimeter based on compact collinear optical arrangement

A miniaturized and high sensitive dual channel fluorimeter was developed and evaluated. It employed collinear optical arrangement, a 365 nm and a 470 nm light emitting diodes (LEDs) as light sources, two photodiodes (PDs) integrated with pre-amplifiers as optoelectronic detectors, and a 12.5 mm × 12.5 mm × 45 mm (width × length × height) quartz cuvette as detection cell. The optical parameters such as spectrum compatibility of dual channel, reshaping lens, the common optical path length (COPL), the common focus lens (CFL), as well as working distance of the cuvette were optimized carefully. It was found that the use of shortened optical path and common focal lens could improve the sensitivity of the dual channel fluorimeter significantly. The limits of detection (LODs) for coumarin, aflatoxin B₁, fluorescein sodium, and vitamin B₂ were 0.002 μg L^-1, 0.006 μg L^-1, 0.008 μg L^-1, and 0.03 μg L^-1, respectively. The dual channel fluorimeter can be used for detection of several categories of substance, such as mycotoxins, polycyclic aromatic hydrocarbons, fluorescein, vitamins, and pathogenic microorganisms etc. As a key component, it can also find application in different disciplines such as fluorescent PCR instruments and 96-well plate fluorescence analyzer.