Related Experiment Video
Updated: Dec 28, 2025
Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
Published on: March 20, 2015
Highly selective detection of l-Phenylalanine by molecularly imprinted polymers coated Au nanoparticles via
Jiayuan Zhou1, Sujitraj Sheth1, Haifeng Zhou1
1Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi, 214122, PR China.
Abstract:
Molecularly imprinted film coated gold nanoprticles (MIP-AuNPs) were employed as surface-enhanced Raman scattering (SERS) substrate for sensitive and selective recognition and quantification of l-Phenylalanine (L-Phe). The MIP was in-situ formed on the AuNPs by sol-gel technique using L-Phe as the template molecule, tetraethyl orthosilicate as the crosslinker and phenyltrimethoxysilane as the functional monomer. The efficient removal of template was achieved by ultrasonic treatment. The as-prepared MIP-AuNPs sensor showed a good linear relationship with the concentration of L-Phe in the range of 1.0 × 10-8-1.0 × 10-4 mol L-1 with a limit of detection as low as 1.0 nmol L-1. The sensor also showed an excellent selectivity as L-Phe can be determined in the presence of other amino acid analogues, D-Phe and bovine serum. The stability of the MIP-AuNPs was manifested by the low deviation (RSD = 3.7%) for 40 days subsequent measurements.
