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Related Experiment Video

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Decellularization and Recellularization of Whole Livers
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Decellularized liver as a translucent ex vivo model for vascular embolization evaluation.

Yanan Gao1, Zhihua Li1, Yin Hong1

  • 1Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong, 518055, China.

Biomaterials
|February 20, 2020
PubMed
Summary

A novel ex vivo liver model aids in evaluating transarterial chemoembolization (TACE) agents. This translucent decellularized liver model reveals how liquid and particle agents perform, guiding future embolic agent development for liver cancer treatment.

Keywords:
Decellularized liver matrixEmbolic agentsInjection pressureTransarterial chemoembolizationVascular system

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Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Oncology

Background:

  • Transarterial chemoembolization (TACE) is a primary treatment for intermediate-stage hepatocellular carcinoma.
  • Current in vitro models lack the complexity of the liver's vasculature for embolic agent development.
  • There is a need for advanced models to accurately assess embolic agent performance in TACE.

Purpose of the Study:

  • To develop a translucent ex vivo liver model for evaluating TACE embolic agents.
  • To investigate the embolization dynamics of liquid and particle-based agents using the novel model.
  • To provide a platform for quantitative characterization of embolization effects.

Main Methods:

  • Decellularization of rat liver organs to create a translucent ex vivo model.
  • Preservation of liver-specific vasculature and enhancement of optical transmittance.
  • Investigation of liquid (ethiodised oil) and particle (poly-dl-lactide, CalliSpheres® beads) embolic agents' performance.

Main Results:

  • The ex vivo model demonstrated improved transmittance (23% at 550 nm) and preserved vasculature.
  • Liquid agents' embolization endpoint depended on injection pressure, risking leakage and damage.
  • Particle agents' embolization endpoint was primarily determined by particle size, with density drop-off being a key factor.

Conclusions:

  • The developed decellularized ex vivo liver model offers a translucent template for TACE agent evaluation.
  • The model effectively differentiates embolization behaviors of liquid versus particle agents.
  • This approach facilitates visual and quantitative assessment of embolotherapy, advancing embolic agent design.