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Related Experiment Video

Updated: Dec 28, 2025

Flow Cytometry Analysis of Immune Cell Subsets within the Murine Spleen, Bone Marrow, Lymph Nodes and Synovial Tissue in an Osteoarthritis Model

Flow Cytometry Analysis of Immune Cell Subsets within the Murine Spleen, Bone Marrow, Lymph Nodes and Synovial Tissue in an Osteoarthritis Model

Published on: April 24, 2020

High-Dimensional Analysis of Postsplenectomy Peripheral Immune Cell Changes.

Jessica S W Borgers^1,2, Richard P Tobin¹, Victoria M Vorwald¹

¹Department of Surgery, School of Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO 80045.

|February 20, 2020

Summary

This summary is machine-generated.

Splenectomy significantly alters human immune cells, increasing lymphocytes and monocytes long-term. These immune changes following spleen removal warrant further investigation into postsplenectomy health outcomes.

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Area of Science:

Immunology
Surgical Oncology
Transplant Immunology

Background:

Human immune responses post-splenectomy are poorly understood compared to animal models.
Elective splenectomy necessitates characterizing circulating immune cell alterations.
Understanding these changes may correlate with postsplenectomy survival.

Purpose of the Study:

To characterize circulating immune cell populations before and after elective splenectomy in humans.
To compare immune changes in splenectomized patients versus those undergoing a Whipple procedure.
To investigate potential relationships between immune alterations and postsplenectomy survival.

Main Methods:

Retrospective analysis of 95 splenectomy patients and 91 Whipple procedure patients.
Single-cell cytometry by time-of-flight mass spectrometry on peripheral blood from five splenectomy patients (pre- and post-surgery).
Detailed characterization of major and minor immune cell populations and chemokine receptor expression.

Main Results:

Splenectomized patients showed sustained elevations in lymphocytes, monocytes, and basophils compared to Whipple procedure patients.
Elevated lymphocytes included naive CD4+ T cells and activated CD25+CD56+CD4+ T cells.
Monocyte elevation comprised mature, activated monocytes, with increased CCR6 and CCR4 expression on multiple cell types.

Conclusions:

Elective splenectomy induces significant and lasting changes in human circulating immune cell populations.
This study provides a detailed comparison of immune changes following splenectomy versus another major abdominal surgery.
The observed immunological shifts highlight the spleen's crucial role in immune homeostasis.