Related Experiment Video

Updated: Dec 28, 2025

Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube
12:48

Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube

Published on: June 2, 2012

17.8K

Cardiac Neural Crest

Hiroyuki Yamagishi1

  • 1Division of Pediatric Cardiology, Department of Pediatrics, Keio University School of Medicine, Shinjuku-ku, Tokyo 160-8582, Japan.

Cold Spring Harbor Perspectives in Biology
|February 20, 2020
PubMed

Insights

Cardiac neural crest (CNC) cells are crucial for heart development, contributing to outflow tract septation. Recent research highlights their signaling with second heart field cells, offering insights into congenital heart diseases.

Area of Science:

  • Developmental biology
  • Cardiovascular research
  • Molecular genetics

Background:

  • Cardiac neural crest (CNC) cells originate from the dorsal neural tube.
  • CNC cells are vital for pharyngeal arch artery remodeling and cardiac outflow tract (OFT) septation.
  • Molecular pathways governing CNC induction, specification, delamination, and migration are complex.

Purpose of the Study:

  • To explore the mechanisms of heart development and disease involving CNC cells.
  • To investigate the role of reciprocal signaling between CNC and second heart field (SHF) cells.
  • To gain new insights into congenital heart diseases (CHDs) and human syndromes related to OFT and aortic arch (AA) development.

Main Methods:

  • Chick ablation models.
  • Molecular biology studies.
  • Analysis of reciprocal signaling pathways.

Main Results:

  • CNC cells contribute significantly to OFT septation and AA remodeling.
  • Reciprocal signaling between CNC and SHF cells is essential for OFT myocardium development.
  • These interactions provide a basis for understanding CHDs.

Conclusions:

  • Understanding CNC cell behavior and their interactions with SHF cells is key to deciphering heart development.
  • This research offers novel perspectives on the molecular underpinnings of CHDs and associated syndromes.

More Related Videos

Analysis of Neural Crest Migration and Differentiation by Cross-species Transplantation09:03

Analysis of Neural Crest Migration and Differentiation by Cross-species Transplantation

Published on: February 7, 2012

23.3K
Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration09:33

Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration

Published on: October 3, 2019

11.4K

Related Concept Videos

Neurulation01:30

Neurulation

Neurulation is the embryological process which forms the precursors of the central nervous system and occurs after gastrulation has established the three primary cell layers of the embryo: ectoderm, mesoderm, and endoderm. In humans, the majority of this system is formed via primary neurulation, in which the central portion of the ectoderm—originally appearing as a flat sheet of cells—folds upwards and inwards, sealing off to form a hollow neural tube. As development proceeds, the...
44.8K
Determination01:51

Determination

During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
20.5K