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Updated: Dec 28, 2025

Methodology for Sputum Induction and Laboratory Processing
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Reliability and Minimum Important Difference of Sputum Weight in Bronchiectasis.

Beatriz Herrero-Cortina1, Victoria Alcaraz-Serrano2, Antoni Torres3

  • 1Universidad San Jorge, Zaragoza, Spain.

Respiratory Care
|February 20, 2020
PubMed
Summary

Sputum weight is a reliable measure for 24-hour collections in bronchiectasis patients. A 17% reduction in sputum post-airway clearance indicates a meaningful improvement for patients.

Keywords:
airway clearance techniquesbronchiectasisminimum important differencepsychometric propertiesreliabilitysputum

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Area of Science:

  • Pulmonary Medicine
  • Respiratory Research
  • Clinical Measurement

Background:

  • Sputum weight is commonly used to evaluate airway clearance interventions in bronchiectasis.
  • However, its psychometric properties, including reliability and minimum important difference, have not been thoroughly assessed.
  • This study addresses this gap by evaluating 24-hour sputum weight in stable bronchiectasis patients.

Purpose of the Study:

  • To determine the test-retest reliability of 24-hour sputum weight in clinically stable individuals with bronchiectasis.
  • To estimate the minimum important difference (MID) of 24-hour sputum weight following an airway clearance session in bronchiectasis patients.

Main Methods:

  • Sixty subjects with bronchiectasis were included for test-retest reliability of 24-hour sputum weight.
  • Forty-two subjects provided sputum weight data before and after airway clearance interventions.
  • Intraclass correlation coefficient (ICC), Bland-Altman analysis, and distribution-based/anchor-based methods were used to assess reliability and MID.

Main Results:

  • Acceptable test-retest reliability for 24-hour sputum weight was found (ICC = 0.75), though agreement was wide for higher sputum volumes.
  • The minimum important difference, calculated via distribution-based methods, was a reduction of 6.4 g (approximately 17% from baseline) in sputum collected 24 hours post-intervention.
  • Anchor-based methods for MID calculation were not feasible due to a lack of correlation between sputum weight and cough impact measures.

Conclusions:

  • For short-term outcome measurement in bronchiectasis, multiple sputum weight collections are recommended to improve agreement.
  • A reduction of 6.4 g (17%) in 24-hour sputum weight post-airway clearance may represent the minimum important difference for patients with bronchiectasis.