Error-free and error-prone DNA repair gene expression data through reprogramming and passage in human iPS cells
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Updated: Dec 28, 2025

Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells iPSCs Using Retroviral Vector with GFP
Published on: April 3, 2012
Yasuhide Yoshimura1, Daisuke Okuzaki2
1Division of Gene Therapy Science, Department of Genome Biology, Graduate School of Medicine, Osaka University, Japan.
Abstract:
We recently found that DNA repair-related gene expression could be altered by reprogramming as well as the increased expression of genes that accurately convey genomic information, such as homologous recombination (HR) and mismatch repair (MMR), and the decreased expression of error-prone translesion synthesis (TLS) polymerase. Here, we confirmed this change in expression in another cell-line and found that such alteration was maintained by overlapping passages as well as OCT3/4 and NANOG. Our findings suggest that changes in the expression of DNA repair-related genes associated with reprogramming and their maintenance can be novel indicators of the quality control of the cells exhibiting pluripotency.
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