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Updated: Dec 28, 2025

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts
Published on: September 13, 2022
[Research progress and applications of gene editing technology CRISPR/Cas in zebrafish]
Juan Ouyang1, Songlei Xue1, Qiqi Zhou1
1Institute of Epigenetics & Epigenomics, Yangzhou University, Yangzhou 225009, Jiangsu, China.
Abstract:
Clustered regularly interspaced short palindromic repeats (CRISPR) are acquired immune system in bacteria and archaea. This system is used in site-directed gene editing. Recently, scientists discovered new CRISPR-associated (Cas) proteins, in which Cas12a-mediated gene editing can significantly reduce the off-target rate. In this article, we review CRISPR/Cas system's discovery of history, composition, classification, and working principle. The latest research progress of the CRISPR/Cas system, and its application in zebrafish are introduced.
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