Related Experiment Video
Updated: Dec 28, 2025
10:19
MicroRNA Expression Profiles of Human iPS Cells, Retinal Pigment Epithelium Derived From iPS, and Fetal Retinal Pigment Epithelium
Published on: June 24, 2014
11.8K
Expression Profiles of MicroRNAs in Stem Cells Differentiation
Hadi Rajabi1, Somayeh Aslani1, Alireza Abhari1
1Department of Biochemistry and Clinical Laboratories, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Current Pharmaceutical Biotechnology
|February 20, 2020
Summary
MicroRNAs regulate stem cell differentiation into functional cells. Understanding these microRNAs can advance regenerative medicine and disease diagnostics.
Area of Science:
- Stem cell biology
- Molecular biology
- Biotechnology
Background:
- Stem cells, including Mesenchymal Stem Cells (MSCs) and Embryonic Stem Cells (ESCs), possess pluripotency and self-renewal capabilities, making them crucial for regenerative medicine.
- Stem cell differentiation into specialized cells is a complex process influenced by signaling pathways and transcription factors.
- MicroRNAs (miRNAs) are increasingly recognized for their roles in disease pathogenesis, cell cycle control, apoptosis, aging, and cell fate determination.
Purpose of the Study:
- To review the regulatory roles of microRNAs in stem cell differentiation, particularly adult stem cells.
- To explore how microRNAs modulate signaling pathways and transcription factors during stem cell differentiation.
- To highlight the potential of understanding miRNA mechanisms for developing stem cell-based therapies and diagnostic biomarkers.
Main Methods:
- Literature review of recent studies on microRNA function in stem cell differentiation.
- Analysis of miRNA expression profiles in different stem cell types.
- Examination of miRNA-mediated regulation of signaling pathways and transcription factors.
Main Results:
- MicroRNAs play a significant role in governing stem cell differentiation into various functional cell types.
- Distinct miRNA expression profiles exist across different stem cell populations.
- MicroRNAs influence stem cell fate decisions by modulating key signaling pathways and transcription factors.
Conclusions:
- MicroRNAs are critical regulators of stem cell differentiation, offering therapeutic potential.
- Elucidating miRNA mechanisms can lead to improved stem cell therapies for regenerative medicine.
- MicroRNAs may serve as valuable biomarkers for early and effective diagnosis of pathological conditions.
More Related Videos
10:48Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
Published on: April 12, 2015
10.5K
08:01Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
Published on: May 30, 2012
10.8K
Related Concept Videos
01:22MicroRNAs
3.6K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.6K
01:22MicroRNAs
23.8K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.8K
01:14Maintenance of the ES Cell State
2.6K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.6K
01:26Stem Cell Niche
6.1K
The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
6.1K
01:18Distinctive Features of Adult Stem Cells vs Cancer Stem Cells
4.3K
A stem cell is an unspecialized cell that can divide without limit as needed and can, under specific conditions, differentiate into specialized cells.
Adult stem cells
Adult stem cells are tissue-specific; hence, they divide to develop the tissue from which they originate. One type of adult stem cell is the epithelial stem cell, which gives rise to the keratinocytes in the multiple layers of epithelial cells in the epidermis of the skin. Adult bone marrow has three distinct types of stem cells:...
Adult stem cells
Adult stem cells are tissue-specific; hence, they divide to develop the tissue from which they originate. One type of adult stem cell is the epithelial stem cell, which gives rise to the keratinocytes in the multiple layers of epithelial cells in the epidermis of the skin. Adult bone marrow has three distinct types of stem cells:...
4.3K