Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

1.6K
Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
1.6K
Ion Exchange01:17

Ion Exchange

1.1K
Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or...
1.1K
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

3.1K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
3.1K
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

1.3K
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
1.3K
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

1.5K
In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
1.5K
Gas Chromatography: Types of Columns and Stationary Phases01:17

Gas Chromatography: Types of Columns and Stationary Phases

2.0K
Gas chromatography (GC) relies on stationary phases to separate and analyze components in a sample. There are two main types of stationary phases: liquid and solid. Liquid stationary phases are non-volatile, thermally stable, and chemically inert liquids coated onto the column. Solid stationary phases are particles of adsorbent material, such as silica gel or molecular sieves.
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
2.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Novel Xanthene Derivatives for Neuroprotection in Alzheimer's Disease-Synthesis and Biological Assessment.

ChemMedChem·2026
Same author

New Bicyclic Sesquiterpene and Labdane Diterpenes from the Culture Extract of the Sea Grass-Derived Fungus <i>Penicillium verruculosum</i> KUFA1509.

Marine drugs·2026
Same author

Benign by design: A paradigm shift in cosmetic ingredient development.

International journal of cosmetic science·2026
Same author

Anti-Inflammatory Evaluation of Pyrazino[2,1-<i>b</i>]quinazoline-3,6-dione Derivatives Inspired by Fiscalin B.

Pharmaceuticals (Basel, Switzerland)·2026
Same author

Exploring cathepsin G inhibition by vinyl-based ionic liquids: Insights from lab-on-valve integrated microsequential analysis and molecular docking approaches.

Analytica chimica acta·2026
Same author

Binding Mode Analysis of Antifouling Compounds Targeting Tyrosinase and Acetylcholinesterase by Saturation Transfer Difference NMR Spectroscopy.

Chembiochem : a European journal of chemical biology·2026

Related Experiment Video

Updated: Dec 28, 2025

From Molecules to Materials: Engineering New Ionic Liquid Crystals Through Halogen Bonding
06:44

From Molecules to Materials: Engineering New Ionic Liquid Crystals Through Halogen Bonding

Published on: March 24, 2018

69.5K

Immobilized imidazolium-based ionic liquids in C18 for solid-phase extraction.

Marieta L C Passos1, Emília Sousa, M Lúcia M F S Saraiva

  • 1LAQV, REQUIMTE, Department of Chemical Sciences, Laboratory of Applied Chemistry, Faculty of Pharmacy, Porto University, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal. marietapassos@gmail.com lsaraiva@ff.up.pt.

The Analyst
|February 20, 2020
PubMed
Summary
This summary is machine-generated.

Two novel solid-phases using immobilized ionic liquids were developed for efficient fluoroquinolone extraction. This eco-friendly UV-irradiation method offers a safer alternative for analyzing antibiotics like norfloxacin and ciprofloxacin.

More Related Videos

Synthesis of Ionic Liquid Based Electrolytes, Assembly of Li-ion Batteries, and Measurements of Performance at High Temperature
11:04

Synthesis of Ionic Liquid Based Electrolytes, Assembly of Li-ion Batteries, and Measurements of Performance at High Temperature

Published on: December 20, 2016

13.3K
Development, Characterization, and Evaluation of CAGE-based Ionic Liquid Systems for Transdermal Delivery
09:44

Development, Characterization, and Evaluation of CAGE-based Ionic Liquid Systems for Transdermal Delivery

Published on: September 26, 2025

343

Related Experiment Videos

Last Updated: Dec 28, 2025

From Molecules to Materials: Engineering New Ionic Liquid Crystals Through Halogen Bonding
06:44

From Molecules to Materials: Engineering New Ionic Liquid Crystals Through Halogen Bonding

Published on: March 24, 2018

69.5K
Synthesis of Ionic Liquid Based Electrolytes, Assembly of Li-ion Batteries, and Measurements of Performance at High Temperature
11:04

Synthesis of Ionic Liquid Based Electrolytes, Assembly of Li-ion Batteries, and Measurements of Performance at High Temperature

Published on: December 20, 2016

13.3K
Development, Characterization, and Evaluation of CAGE-based Ionic Liquid Systems for Transdermal Delivery
09:44

Development, Characterization, and Evaluation of CAGE-based Ionic Liquid Systems for Transdermal Delivery

Published on: September 26, 2025

343

Area of Science:

  • Analytical Chemistry
  • Environmental Chemistry
  • Materials Science

Background:

  • Fluoroquinolones are widely used antibiotics with environmental and health concerns.
  • Conventional solid-phase extraction methods may involve toxic reagents.
  • Development of safer and efficient extraction techniques is crucial.

Purpose of the Study:

  • To synthesize and characterize novel solid-phases for fluoroquinolone extraction.
  • To develop an environmentally friendly immobilization process using UV-irradiation.
  • To evaluate the efficiency of these solid-phases for extracting specific fluoroquinolones.

Main Methods:

  • Immobilization of imidazolium-based ionic liquids onto solid supports via UV-irradiation.
  • Characterization of the synthesized solid-phases using nuclear magnetic resonance spectroscopy and elemental analysis.
  • Optimization of solid-phase extraction parameters, including elution solvent, eluent volume, and sample loading volume.

Main Results:

  • Two distinct solid-phases with immobilized ionic liquids were successfully prepared.
  • The immobilization process was confirmed to be non-toxic and efficient.
  • The developed solid-phases demonstrated successful extraction of norfloxacin and ciprofloxacin.
  • Optimized parameters led to effective separation and recovery of the target analytes.

Conclusions:

  • The developed solid-phases offer a promising, eco-friendly alternative for fluoroquinolone extraction.
  • The method shows high potential for the analysis of other fluoroquinolone antibiotics.
  • This approach contributes to greener analytical chemistry practices.