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LncRNA-ZNF281 Interacts with miR-539 to Promote Hepatocellular Carcinoma Cell Invasion and Migration
Zhibo Zhang1, Lanfang Yang1, Xiaongqing Yao1
1Department of Hepatopancreas, Biliary Surgery, and The First Affiliated Hospital of Fujian Medical University, Fuzhou City, P.R. China.
Abstract:
LncRNA-ZNF281 suppresses glioma, whereas its role in hepatocellular carcinoma (HCC) is unclear. This study aimed to investigate the interaction between ZNF281 and miR-539 in HCC. This study included 66 HCC patients (40 men and 26 women; 36-68 years, 53.1 ± 6.2 years) who were selected from the 133 HCC cases admitted to The First Affiliated Hospital of Fujian Medical University from February 2011 to June 2014. Levels of ZNF281 and miR-539 expression in two types of tissues (HCC and nontumor) were measured by performing quantitative polymerase chain reaction (qPCR). Dual-luciferase assay was performed to analyze the interactions between miR-539 and ZNF281 in both SNU-475 and PLHC-1 cells. The effects of ZNF281 and miR-539 overexpression on the invasion and migration of HCC cells were analyzed by performing transwell assays. The authors showed that ZNF281 was upregulated and miR-539 was downregulated in HCC tissues and were negatively correlated. High levels of ZNF281 and low levels of miR-539 predicted the poor survival of HCC patients. Overexpression analysis showed that ZNF281 and miR-539 overexpression led to the downregulation of each other. Transwell assays showed that ZNF281 overexpression led to enhanced and miR-539 overexpression led to suppressed HCC cell invasion and migration. In addition, miR-539 overexpression attenuated the effects of ZNF281 overexpression. Therefore, ZNF281 may interact with miR-539 to promote HCC cell invasion and migration.
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