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Related Concept Videos

The Mitotic Spindle02:27

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Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
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Spindle pole bodies function as signal amplifiers in the Mitotic Exit Network.

Ian W Campbell1, Xiaoxue Zhou1, Angelika Amon1

  • 1David H. Koch Institute for Integrative Cancer Research, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139.

Molecular Biology of the Cell
|February 20, 2020
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Summary

The Mitotic Exit Network (MEN) in yeast uses spindle pole bodies (SPBs) to link nuclear position to cell division completion. Scaffolding MEN onto SPBs ensures timely cell cycle progression by amplifying signals.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • The Mitotic Exit Network (MEN) is a crucial signal transduction cascade in budding yeast.
  • MEN regulates the transition from mitosis to cell cycle exit.
  • Nuclear position is a key factor influencing MEN activity.

Purpose of the Study:

  • To investigate how scaffolding the MEN onto spindle pole bodies (SPBs) couples mitosis exit to nuclear position.
  • To determine the relative importance of MEN signaling from the daughter cell SPB (dSPB) versus the mother cell SPB.
  • To elucidate the mechanism by which MEN components localize and function during cell division.

Main Methods:

  • Quantitative analysis of MEN component localization.
  • Microscopy techniques to observe SPB and nuclear positioning.
  • Genetic manipulation of MEN components and SPB function.

Main Results:

  • Scaffolding MEN onto SPBs links final mitotic stages to spindle position.
  • Active Tem1 GTPase accumulates at the dSPB when the nucleus moves into the bud.
  • MEN kinase cascade components localize to both SPBs, promoting efficient mitosis exit.

Conclusions:

  • MEN signaling is initiated by Tem1 at the dSPB.
  • Localization of MEN kinases to both SPBs amplifies signaling for timely mitosis exit.
  • Cells with a single SPB exhibit delayed exit from mitosis.