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Related Concept Videos

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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Detection of Histone Modifications in Plant Leaves
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DNA Extraction from Plant Leaves Using a Microneedle Patch.

Rajesh Paul1, Emily Ostermann1, Zhen Gu2,3

  • 1Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina.

Current Protocols in Plant Biology
|February 20, 2020
PubMed
Summary
This summary is machine-generated.

A novel microneedle (MN) patch rapidly isolates plant DNA from leaf tissues within a minute. This method simplifies sample preparation for on-site plant pathogen detection without extensive laboratory equipment.

Keywords:
DNA extractionMN patchPCRin-field diagnosticsmicroneedle patchplant pathogen

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Area of Science:

  • Plant Pathology
  • Molecular Biology
  • Biotechnology

Background:

  • Effective DNA isolation from plant tissues is crucial for molecular plant pathogen detection.
  • Current methods are complex, require laboratory equipment, and hinder on-site diagnostics.
  • Developing rapid, field-deployable DNA extraction techniques is essential.

Purpose of the Study:

  • To develop a simple, rapid, and field-deployable DNA extraction method for plant tissues.
  • To overcome the limitations of traditional laboratory-based DNA isolation for plant pathogen detection.
  • To enable on-site sample preparation for molecular diagnostics.

Main Methods:

  • A microneedle (MN) patch fabricated from polyvinyl alcohol (PVA) was utilized for DNA extraction.
  • The MN patch penetrates plant leaf tissues, breaking cell walls to release intracellular DNA.
  • The extracted DNA was directly used for Polymerase Chain Reaction (PCR) amplification without purification.

Main Results:

  • The MN patch successfully isolated plant DNA from various plant species within one minute.
  • Extracted DNA was PCR-amplifiable, demonstrating high quality.
  • The method effectively isolated Phytophthora infestans DNA from infected tomato leaves.
  • The MN patch showed potential for direct DNA isolation from plant pathogens in the field.

Conclusions:

  • The microneedle patch offers a minimally invasive and efficient method for plant DNA extraction.
  • This technique significantly simplifies sample preparation for on-site plant pathogen detection.
  • The MN patch has broad applicability for rapid molecular diagnostics in diverse plant species and pathogens.