Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Introduction to Plant Diversity

Introduction to Plant Diversity

From Water to Land

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Comparative prognostic analysis of primary thymic adenocarcinoma and squamous cell carcinoma: a 20-year retrospective real-world study.

Journal of thoracic disease·2026

Same author

Genome-wide identification and expression profiling of the NHX gene family in oat (Avena sativa L.).

BMC genomics·2026

Same author

Hierarchical Porous P-Doped NiCo Alloy with α/ε Phase-Defect Synergy to Boost Alkaline HER Kinetics and Bifunctional Activity.

Nanomaterials (Basel, Switzerland)·2025

Same author

Efficient Conversion for Biofuels: From Lignin Modification to Enzymatic Delignification of Plant Cell Wall.

Plant, cell & environment·2025

Same author

Study on the relationship between medical staff's knowledge, attitudes, and practices regarding medical waste classification and personality traits.

Frontiers in health services·2025

Same author

Epicatechin Gallate Regulation of Steroid Hormone Levels Improves Sarcopenia in C57BL/6J Mice.

Foods (Basel, Switzerland)·2025

Search research articles

Related Experiment Video

Updated: Dec 28, 2025

Poplar Adventitious Roots Induced by Stem Canker Pathogens: An Experimental System for Studying Roots Biology and Light Response-Related Processes

Poplar Adventitious Roots Induced by Stem Canker Pathogens: An Experimental System for Studying Roots Biology and Light Response-Related Processes

Published on: October 11, 2024

The bZIP53-IAA4 module inhibits adventitious root development in Populus.

Yan Zhang¹, Xiaoqing Yang¹, Pei Cao¹

¹College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, China.

Journal of Experimental Botany

|February 21, 2020

Summary

A novel salt-responsive gene module, bZIP53-IAA4, negatively regulates adventitious root (AR) development in poplar. This discovery offers insights into plant adaptation to salt stress and clonal propagation strategies.

Keywords:

IAA4 Populus Adventitious roots Aux/IAA genes bZIP transcription factors bZIP53 salt responsive genes

More Related Videos

The Use of Induced Somatic Sector Analysis ISSA for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development

The Use of Induced Somatic Sector Analysis ISSA for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development

Published on: October 5, 2016

Author Spotlight: High-Throughput In Vivo Leaf Inoculation for Accelerating Disease Resistance Screening in Poplar Hybrid Breeding

Author Spotlight: High-Throughput In Vivo Leaf Inoculation for Accelerating Disease Resistance Screening in Poplar Hybrid Breeding

Published on: September 20, 2024

Related Experiment Videos

Last Updated: Dec 28, 2025

Poplar Adventitious Roots Induced by Stem Canker Pathogens: An Experimental System for Studying Roots Biology and Light Response-Related Processes

Poplar Adventitious Roots Induced by Stem Canker Pathogens: An Experimental System for Studying Roots Biology and Light Response-Related Processes

Published on: October 11, 2024

The Use of Induced Somatic Sector Analysis ISSA for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development

The Use of Induced Somatic Sector Analysis ISSA for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development

Published on: October 5, 2016

Author Spotlight: High-Throughput In Vivo Leaf Inoculation for Accelerating Disease Resistance Screening in Poplar Hybrid Breeding

Author Spotlight: High-Throughput In Vivo Leaf Inoculation for Accelerating Disease Resistance Screening in Poplar Hybrid Breeding

Published on: September 20, 2024

Area of Science:

Plant Biology
Molecular Genetics
Plant Physiology

Background:

Adventitious roots (ARs) are crucial for clonal propagation in many plant species.
Understanding the genetic regulation of AR development is key to improving plant propagation techniques.

Purpose of the Study:

To identify and characterize a salt-responsive gene module involved in the negative regulation of AR development in poplar.
To elucidate the molecular mechanism of the bZIP53-IAA4 module in controlling AR growth.

Main Methods:

Gene expression analysis (RNA-seq, RT-qPCR) in poplar and Arabidopsis.
Functional analysis of bZIP53, IAA4-1, and IAA4-2 through overexpression and induced expression.
Protein-DNA interaction assays (yeast one-hybrid, electrophoretic mobility shift, dual luciferase reporter, GUS co-expression).

Main Results:

Salt stress induces bZIP53 expression, a transcription factor that inhibits AR growth.
bZIP53 directly regulates the downstream genes IAA4-1 and IAA4-2.
Overexpression of bZIP53, IAA4-1, or IAA4-2 leads to inhibited AR development in poplar and Arabidopsis.
Both bZIP53 and IAA4-1/2 genes respond to salt stress in poplar.

Conclusions:

The bZIP53-IAA4 gene module acts as a negative regulator of adventitious root development in poplar.
This module plays a role in poplar's response to salt stress.
Findings contribute to understanding the genetic control of AR formation and salt tolerance.