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Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
Published on: March 19, 2018
Identification of Dysfunctional Gut Microbiota Through Rectal Swab in Patients with Different Severity of Acute
Shanshan Yu1, Yangyang Xiong2, Jun Xu1
1Department of Emergency Medicine, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, No. 1 Shuaifuyuan, Dongcheng District, Beijing, 100730, China.
Background:
Acute pancreatitis (AP) has a wide spectrum of severity and can be associated with considerable morbidity and mortality. Whether gut microbiota dysbiosis is associated with AP severity remains obscure.
Aims:
We aim to investigate the differences in the alterations of gut microbiota in different grades of AP severity.
Methods:
We collected clinical information and rectal swab samples from 80 individuals. The gut microbiota was tested by 16S rRNA gene sequencing, gut microbiota species composition analysis, difference analysis, random forest model prediction analysis, and gut microbiota species correlation network analysis.
Results:
There was a different microbiota profile in different severity grades. Bacteroides, Escherichis-Shigella, and Enterococcus were dominant species in mild, moderately severe, and severe AP, respectively. Finegoldia was the most significantly increased and Blautia the most decreased species in mild AP. Anaerococcus was the most significantly increased and Eubacterium hallii the most decreased species in moderately severe AP. Enterococcus was the most significantly increased and Eubacterium hallii the most decreased species in severe AP. Finegoldia, Eubacterium_hallii, and Lachnospiraceae were potential diagnostic biomarkers for mild AP and Eubacterium_hallii and Anaerococcus for moderately severe AP. There was a positive interaction between Firmicutes and Bacteroidetes in mild AP.
Conclusions:
The disturbed gut microbiota is different among grades of AP, suggesting their potential role in the progression of disease severity. There was a different microbiota profile in different severity grades. Bacteroides, Escherichis-Shigella, and Enterococcus were dominant gut microbiota species in MAP, MSAP, and SAP, respectively. Finegoldia was the most significantly increased and Blautia the most decreased gut microbiota species in MAP. Anaerococcus was the most significantly increased and Eubacterium hallii the most decreased species in MSAP. Enterococcus was the most significantly increased and Eubacterium hallii the most decreased species in SAP. Finegoldia, Eubacterium_hallii, and Lachnospiraceae were potential diagnostic biomarkers for MAP and Eubacterium_hallii and Anaerococcus for MSAP. There was a positive interaction between Firmicutes and Bacteroidetes in MAP.
Insights
Gut microbiota profiles differ significantly across acute pancreatitis (AP) severity grades. Specific bacterial species like Bacteroides, Escherichis-Shigella, and Enterococcus are dominant in mild, moderate, and severe AP, respectively, indicating a role in disease progression.
Area of Science:
- Gastroenterology
- Microbiology
- Genomics
Background:
- Acute pancreatitis (AP) presents a broad spectrum of severity, often leading to significant morbidity and mortality.
- The association between gut microbiota dysbiosis and AP severity remains unclear.
Purpose of the Study:
- To investigate distinct gut microbiota alterations in different grades of AP severity.
- To identify potential microbial biomarkers for AP severity classification.
Main Methods:
- Collected clinical data and rectal swab samples from 80 individuals.
- Utilized 16S rRNA gene sequencing for gut microbiota analysis.
- Performed species composition, difference, random forest, and correlation network analyses.
Main Results:
- Distinct gut microbiota profiles were observed across AP severity grades.
- Dominant species varied: Bacteroides (mild AP), Escherichis-Shigella (moderately severe AP), and Enterococcus (severe AP).
- Identified potential diagnostic biomarkers: Finegoldia, Eubacterium_hallii, and Lachnospiraceae for mild AP; Eubacterium_hallii and Anaerococcus for moderately severe AP.
Conclusions:
- Disturbed gut microbiota composition correlates with AP severity, suggesting a role in disease progression.
- Specific bacterial species and their interactions may serve as indicators for AP severity.
- Further research into these microbial signatures could inform diagnostic and therapeutic strategies.
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