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PP4R1 interacts with HMGA2 to promote non-small-cell lung cancer migration and metastasis via activating

Bin Wang1, Lin-Yue Pan2, Ning Kang1

  • 1Department of Thoracic Surgery, The Affiliated Huadong Hospital of Fudan University, Shanghai, China.

Molecular Carcinogenesis
|February 21, 2020
PubMed

Insights

Protein phosphatase 4 regulatory subunit 1 (PP4R1) is upregulated in non-small-cell lung cancer, promoting cell migration and invasion. This study identifies PP4R1 as a potential oncogene and therapeutic target for lung cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Protein phosphatase 4 regulatory subunit 1 (PP4R1) is involved in cell cycle regulation, DNA repair, and apoptosis.
  • The specific role of PP4R1 in non-small-cell lung cancer (NSCLC) pathogenesis is not well understood.

Purpose of the Study:

  • To investigate the role and clinical significance of PP4R1 in non-small-cell lung cancer.
  • To elucidate the underlying molecular mechanisms by which PP4R1 influences NSCLC progression.

Main Methods:

  • Oncomine database mining and immunohistochemical staining to assess PP4R1 expression in NSCLC tissues.
  • In vitro assays (wound-healing, Transwell) to evaluate the effect of PP4R1 on NSCLC cell migration and invasion.
  • Mechanistic studies to explore the interaction of PP4R1 with other proteins and signaling pathways (MAPK/ERK).

Main Results:

  • PP4R1 is significantly upregulated in NSCLC tissues compared to normal lung tissues.
  • High PP4R1 expression correlates with poor prognosis in NSCLC patients.
  • PP4R1 overexpression promotes NSCLC cell migration and invasion.
  • PP4R1 cooperates with high mobility group AT-hook 2 to drive epithelial-mesenchymal transition via MAPK/ERK activation.

Conclusions:

  • PP4R1 functions as an oncogene in non-small-cell lung cancer.
  • PP4R1 promotes NSCLC progression by enhancing cell migration, invasion, and epithelial-mesenchymal transition.
  • PP4R1 represents a potential diagnostic biomarker and therapeutic target for NSCLC.
Keywords:
ERKPP4R1epithelial-mesenchymal transitionmigration and metastasisnon-small-cell lung cancer

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