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Transmembrane ferricyanide reductase activity in Ehrlich ascites tumor cells
M A Medina1, F Sánchez-Jiménez, J A Segura
1Laboratorio de Bioquímica, Facultad de Ciencias, Universidad de Málaga, Spain.
Biochimica Et Biophysica Acta
|December 8, 1988
Summary
Ehrlich ascites tumor cells exhibit transmembrane ferricyanide reductase activity, influenced by lactate and amiloride. Cytotoxic drugs inhibit this redox activity, suggesting a potential therapeutic target for cancer treatment.
Area of Science:
- Biochemistry
- Cell Biology
- Oncology
Background:
- Ehrlich ascites tumor cells possess a transmembrane ferricyanide reductase activity.
- This activity is linked to cytosolic NADH availability and proton extrusion.
Purpose of the Study:
- To characterize the kinetics and regulation of transmembrane ferricyanide reductase in Ehrlich ascites tumor cells.
- To investigate the effect of cytotoxic drugs on this enzymatic activity.
Main Methods:
- Assay of transmembrane ferricyanide reductase activity in intact Ehrlich ascites tumor cells.
- Kinetic measurements (Km, Vmax) were performed.
- Effects of lactate, amiloride, and cytotoxic drugs were evaluated.
Main Results:
- Km of 0.14 mM and Vmax of 0.31 μmol/min per 10^6 cells were determined.
- Lactate enhanced the activity, while amiloride diminished it.
- Cytotoxic drugs significantly inhibited the reductase activity at therapeutic concentrations.
Conclusions:
- Transmembrane ferricyanide reductase activity is a key feature of Ehrlich ascites tumor cells.
- This activity is modulated by cellular metabolites and ion transport mechanisms.
- Inhibition of this reductase by cytotoxic drugs indicates its potential role in antineoplastic effects.