Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Molecular Engineering for Nonlinear Fluorescence: En Route to Three-Photon Absorption via Sequential One-Photon Excitation.

Journal of the American Chemical Society·2026

Same author

Room temperature collective blinking and photon bunching from CsPbBr<sub>3</sub> quantum dot superlattice.

Nature communications·2026

Same author

Bifunctional polymer assisted growth of crack-free thick perovskite films for flexible X-ray detection.

Journal of materials chemistry. C·2026

Same author

ABEL-FRET bridges the timescale gap in single-molecule measurements of the structural dynamics in the A<sub>2A</sub> adenosine receptor.

Communications chemistry·2026

Same author

Harnessing Methyltransferase-Guided Targeting for Sequence-Specific Proximity Labeling of DNA.

Angewandte Chemie (International ed. in English)·2026

Same author

Suppressed Photoluminescence Blinking and Enhanced Stability in Ligand-Engineered Pure Blue-Emitting Perovskite Nanocrystals.

ACS nano·2026

Search research articles

Related Experiment Video

Updated: Dec 28, 2025

Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues

Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues

Published on: November 22, 2014

Fluorescent SAM analogues for methyltransferase based DNA labeling.

Vince Goyvaerts¹, Sven Van Snick¹, Laurens D'Huys¹

¹Laboratory of Molecular Imaging and Photonics, Department of Chemistry, KU Leuven, Celestijnenlaan 200F, 3001 Leuven, Belgium. johan.hofkens@kuleuven.be.

Chemical Communications (Cambridge, England)

|February 21, 2020

Summary

New S-adenosyl-l-methionine (SAM) analogues using cysteine were synthesized for DNA labeling. These fluorescent cofactors enable methyltransferase-based optical mapping across the visible spectrum.

More Related Videos

Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments

Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments

Published on: December 21, 2017

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Published on: August 26, 2009

Related Experiment Videos

Last Updated: Dec 28, 2025

Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues

Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues

Published on: November 22, 2014

Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments

Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments

Published on: December 21, 2017

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Published on: August 26, 2009

Area of Science:

Biochemistry
Organic Chemistry
Molecular Biology

Background:

S-adenosyl-l-methionine (SAM) is a crucial biological cofactor involved in methyl group transfer.
Current methods for DNA labeling can be labor-intensive and lack versatility.
Sequence-specific DNA labeling is essential for various molecular biology applications.

Purpose of the Study:

To develop novel S-adenosyl-l-methionine (SAM) analogues for efficient and sequence-specific DNA labeling.
To create fluorescent SAM analogues with emission across the visible spectrum.
To demonstrate the utility of these analogues in methyltransferase-based optical mapping.

Main Methods:

Synthesis of non-natural SAM analogues incorporating cysteine instead of the natural homolog.
Utilizing readily available starting materials for near quantitative conversion.
Generation of fluorescent cofactors spanning the visible spectrum.

Main Results:

Successful preparation of novel SAM analogues with high synthetic efficiency.
Development of fluorescent cofactors with diverse spectral properties.
Demonstrated applicability of the synthesized analogues in methyltransferase-based optical mapping.

Conclusions:

The developed synthetic strategy provides a robust method for creating diverse SAM analogues.
These novel SAM analogues are effective tools for sequence-specific DNA labeling.
The fluorescent cofactors show significant potential for advancing optical mapping techniques.