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The HOPS complex subunit VPS39 controls ciliogenesis through autophagy.

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Vacuolar protein sorting 39 (VPS39) negatively regulates primary cilia formation by controlling protein transport via autophagy. This discovery in human cells and fish offers a potential new target for treating kidney diseases linked to cilia dysfunction.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Renal Physiology

Background:

  • Primary cilia are crucial microtubule-based organelles involved in cellular signaling.
  • Cilia dysfunction causes ciliopathies, often featuring renal cystic disease.
  • The role of autophagy in ciliogenesis is increasingly recognized.

Purpose of the Study:

  • To investigate the role of vacuolar protein sorting 39 (VPS39) in ciliogenesis.
  • To elucidate the mechanism by which VPS39 regulates cilia formation.
  • To explore VPS39 as a potential therapeutic target for ciliopathies.

Main Methods:

  • Utilized human renal cells and medaka fish models.
  • Investigated the localization of intraflagellar transport proteins.
  • Examined the interplay between VPS39, autophagy, and ciliogenesis.

Main Results:

  • VPS39 acts as a negative regulator of ciliogenesis in human renal cells.
  • VPS39 controls ciliogenesis via autophagy by affecting protein localization at the ciliary base.
  • VPS39-mediated regulation of ciliogenesis occurs in vivo in medaka fish renal tubules.
  • Autophagy's impact on ciliogenesis is cell-type and stimulus-dependent.

Conclusions:

  • The homotypic fusion and vacuole protein sorting (HOPS) complex, via VPS39, directly regulates autophagy-mediated ciliogenesis.
  • VPS39 is a novel biological target for potentially recovering cilia-related kidney phenotypes in ciliopathies.