Related Experiment Video

Updated: Dec 28, 2025

Author Spotlight: Developing Tools to Tune the Activity of Tyrosine Phosphatases
06:56

Author Spotlight: Developing Tools to Tune the Activity of Tyrosine Phosphatases

Published on: September 6, 2024

672

Target Of Rapamycin pathway in the white-rot fungus Phanerochaete chrysosporium

Duy Vuong Nguyen1, Thomas Roret1, Antonio Fernandez-Gonzalez1

  • 1Université de Lorraine, INRA, IAM, France.

Plos One
|February 21, 2020
PubMed

Insights

The Target Of Rapamycin (TOR) pathway regulates growth in the fungus Phanerochaete chrysosporium. Inhibiting TOR with rapamycin impacts fungal development and protein secretion, showing its crucial role.

Area of Science:

  • Mycology
  • Molecular Biology
  • Biochemistry

Background:

  • The Target Of Rapamycin (TOR) signaling pathway is a crucial regulator of cell growth and metabolism in eukaryotes.
  • Nutrient availability and stress conditions are key environmental factors influencing TOR pathway activity.
  • Understanding TOR signaling in fungi is vital for comprehending their growth and response mechanisms.

Purpose of the Study:

  • To investigate the role and components of the TOR signaling pathway in the white-rot fungus Phanerochaete chrysosporium.
  • To determine the effects of TOR inhibition on fungal growth and development.
  • To identify homologs of TOR pathway components within the P. chrysosporium genome.

Main Methods:

  • Treatment of P. chrysosporium with rapamycin to inhibit TOR activity.
  • Assessment of conidia germination and hyphal growth.
  • Analysis of secreted protein content.
  • Bioinformatic identification of TOR pathway component homologs.

Main Results:

  • Rapamycin treatment significantly affected conidia germination and hyphal growth in P. chrysosporium.
  • Inhibition of TOR signaling altered the profile of secreted proteins.
  • Homologs of key TOR pathway components were identified in P. chrysosporium.
  • The results demonstrate a conserved susceptibility to rapamycin.

Conclusions:

  • The TOR signaling pathway plays a central and conserved role in regulating growth and development in Phanerochaete chrysosporium.
  • TOR signaling influences not only growth but also the secretome of this fungus.
  • The findings provide insights into the molecular mechanisms governing fungal responses to nutrient and stress signals.

More Related Videos

A Semi-Quantitative Drug Affinity Responsive Target Stability DARTS assay for studying Rapamycin/mTOR interaction05:28

A Semi-Quantitative Drug Affinity Responsive Target Stability DARTS assay for studying Rapamycin/mTOR interaction

Published on: August 27, 2019

17.4K
Analysis of Autophagy in Penicillium chrysogenum by Using Starvation Pads in Combination With Fluorescence Microscopy06:51

Analysis of Autophagy in Penicillium chrysogenum by Using Starvation Pads in Combination With Fluorescence Microscopy

Published on: February 1, 2015

8.9K

Related Concept Videos

PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
5.1K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.5K
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
7.7K
cAMP-dependent Protein Kinase Pathways01:25

cAMP-dependent Protein Kinase Pathways

Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
8.0K
Riboswitches01:56

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
9.4K
The Unfolded Protein Response01:37

The Unfolded Protein Response

The ER is the hub of protein synthesis in a cell. It has robust systems to quality control protein folding and also for degradation of terminally misfolded proteins. Under normal conditions, a small proportion of misfolded proteins that cannot be salvaged need to be transported to the cytoplasm by the ER-associated degradation or ERAD pathways. However, if the ERAD cannot handle the misfolded proteins, the cell activates the unfolded protein response or UPR to adjust the protein folding...
6.1K