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Mechanisms and Regulation of Nonsense-Mediated mRNA Decay and Nonsense-Associated Altered Splicing in Lymphocytes

Jean-Marie Lambert1, Mohamad Omar Ashi1, Nivine Srour1,2

  • 1Unit CNRS 7276 - INSERM U1262 - Limoges University, 2 rue du Docteur Marcland, 87025 Limoges, France.

International Journal of Molecular Sciences
|February 22, 2020
PubMed

Insights

Cells eliminate dangerous truncated proteins using RNA surveillance. Nonsense-mediated mRNA decay (NMD) and nonsense-associated altered splicing (NAS) degrade or correct faulty messenger RNAs (mRNAs) containing premature termination codons (PTCs).

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Premature termination codons (PTCs) create unstable transcripts encoding harmful proteins.
  • Cells employ RNA surveillance to prevent the synthesis of truncated proteins.
  • Nonsense-mediated mRNA decay (NMD) and nonsense-associated altered splicing (NAS) are key RNA quality control pathways.

Purpose of the Study:

  • To review the mechanisms of NMD and NAS.
  • To explore the role of NMD and NAS in lymphocyte immunoglobulin and TCR transcript regulation.

Main Methods:

  • Literature review of NMD and NAS pathways.
  • Analysis of RNA surveillance in lymphocyte gene expression.

Main Results:

  • NMD targets PTC-containing mRNAs for degradation.
  • NAS promotes alternative splicing to bypass PTCs, upregulating functional transcripts.
  • Both pathways protect cells from deleterious truncated proteins.

Conclusions:

  • NMD and NAS are crucial RNA quality control systems.
  • These pathways eliminate aberrant transcripts, maintaining cellular integrity.
  • Understanding NMD and NAS is vital for lymphocyte biology and disease research.
Keywords:
Immunoglobulin (Ig), Nonsense-mediated mRNA decay (NMD), Nonsense-associated altered splicing (NAS), B lymphocytesPlasma cells

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