Characteristics of sewer biofilms in aerobic rural small diameter gravity sewers

Small diameter gravity sewers (SDGS) are extensively used to collect rural sewage as they are low in cost and quick to construct. However, the characteristics of biofilms in rural SDGS are still not clear. In this study, biofilms characteristics of aerobic rural SDGS were investigated using simulations in a lab under different flow conditions and slopes. Results indicated that the average thickness of aerobic rural SDGS biofilms was in the range of 350-650 μm, decreasing at locations with variable flow and high slopes. Protein was the most abundant substance in extracellular polymeric substance of SDGS biofilms. The most abundant bacteria, Proteobacteria, Actinobacteria, and Bacteroidetes, and functional bacteria showed different distributions when analyzed through Illumina HiSeq sequencing of 16S rRNA. The relative abundances of denitrifying bacteria, nitrite-oxidizing bacteria, and sulfate-reducing bacteria (SRB) were lower during variable flow than during stable flow. High slopes (15‰) decreased SRB presence, which could be used to mitigate H₂S accumulation in aerobic SDGS. Overall, this study describes the characteristics of aerobic rural SDGS biofilms and provides valuable suggestions for the optimal design of SDGS based on these characteristics.