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Updated: Dec 28, 2025

Ex Vivo Analysis of Mechanically Activated Ca2+ Transients in Urothelial Cells
Published on: September 28, 2022
Composite proportional-integral sliding mode control with feedforward control for cell puncture mechanism with
Shengdong Yu1, Mingyang Xie2, Hongtao Wu2
1Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China; Wenzhou Vocational and Technical College, Wenzhou 325000, China.
Abstract:
This paper presents a novel control strategy to compensate hysteretic nonlinearity and achieve precise positioning control of a cell puncture mechanism driven by a piezoelectric actuator (PEA). A dynamic model of the cell puncture mechanism is developed based on the Bouc-Wen model. Parameters of the nonlinear model are identified by particle swarm optimization. The strategy of feedforward (FF) control and sliding mode feedback (FB) control based on the Bouc-Wen inverse model is further developed to position the cell puncture mechanism. Zebrafish embryo is used as the validation object, wherein a cell micropuncture experiment is successfully performed. Proportional-integral sliding mode FB control plus FF control has a simple structure and exhibits excellent performance. Thus, this method can be easily extended to other micro-or nanopositioning mechanisms based on PEAs and adopted in practical applications.
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