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Expression pattern of Nav2 in the murine CNS with development.

Caitlin Pook1, Jamie M Ahrens2, Margaret Clagett-Dame3

  • 1Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison, WI, 53706, USA; Medical College of Wisconsin-Milwaukee Campus, Wauwatosa, WI, 53226, USA.

Gene Expression Patterns : GEP
|February 22, 2020
PubMed
Summary

Neuron navigator 2 (NAV2) full-length transcript is the predominant form in the mouse central nervous system (CNS). NAV2 expression is highest during embryonic and early postnatal development, coinciding with neuronal migration and differentiation.

Keywords:
All-trans retinoic acidAxon elongationCNS developmentCell migrationNeuron navigator

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Molecular Biology

Background:

  • Neuron navigator 2 (NAV2) is crucial for nervous system development.
  • NAV2 has multiple predicted protein forms due to alternative splicing and promoter usage.
  • Understanding NAV2 transcript distribution is key to elucidating its developmental roles.

Purpose of the Study:

  • To investigate the spatial and temporal distribution of Nav2 transcripts in the mouse central nervous system (CNS).
  • To determine the predominant Nav2 transcript form in the developing and adult mouse CNS.
  • To correlate Nav2 expression patterns with neuronal migration and differentiation processes.

Main Methods:

  • In situ hybridization using 5' and 3' Nav2 probes.
  • Real-time quantitative PCR (qPCR) with primers spanning the Nav2 mRNA.
  • Combined in situ hybridization with immunohistochemistry (Ki67, CTIP2, Nissl staining).
  • Dual fluorescent probe in situ hybridization.

Main Results:

  • Full-length Nav2 transcript is the predominant form in the wild-type mouse CNS.
  • High Nav2 expression observed in developing cortex, hippocampus, olfactory bulb, and cerebellar granule cells.
  • Expression peaks during late gestation and early postnatal life, correlating with neuronal migration and differentiation.
  • A hypomorphic mouse model shows limited Nav2 expression, primarily shorter transcripts in oligodendrocytes at the cerebellum's base.

Conclusions:

  • Full-length Nav2 transcript is the primary form across numerous mouse CNS regions.
  • NAV2 plays a significant role in CNS development beyond its known cerebellar functions.
  • Nav2 expression patterns support its involvement in neuronal migration and differentiation during development.