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A Macintosh Hypercard stack for calculation of thermodynamically-corrected buffer recipes.

R J Beynon1

  • 1Department of Biochemistry, University of Liverpool, UK.

Computer Applications in the Biosciences : CABIOS
|November 1, 1988
PubMed
Summary
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This study presents a Macintosh HyperCard stack for calculating thermodynamically-corrected pH buffers. The software offers comprehensive buffer design, enabling precise control over buffer species, temperature, and ionic strength for accurate experimental conditions.

Area of Science:

  • Biochemistry
  • Chemical Engineering
  • Software Development

Background:

  • Accurate pH control is crucial for many biochemical and chemical processes.
  • Traditional methods for buffer calculation can be complex and time-consuming.
  • Existing software may lack flexibility in controlling buffer parameters.

Purpose of the Study:

  • To develop a user-friendly software tool for calculating thermodynamically-corrected pH buffers.
  • To provide researchers with precise control over buffer composition and experimental conditions.
  • To simplify the process of buffer design and recipe generation.

Main Methods:

  • Development of a stack for the Apple Macintosh HyperCard environment.
  • Implementation of algorithms for thermodynamically-corrected pH buffer calculations.

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  • Inclusion of user-defined parameters for buffer species, temperature, ionic strength, and counter-ion selection.

    • Main Results:

    • The stack facilitates the calculation of thermodynamically-corrected pH buffers.
    • Users can comprehensively design buffers with full control over key parameters.
    • Generated recipes include precise component weights for easy preparation.

    Conclusions:

    • The developed Macintosh HyperCard stack offers a powerful and flexible tool for buffer design.
    • This software simplifies the creation of accurate pH buffers, enhancing experimental reproducibility.
    • The straightforward interface and detailed output facilitate efficient laboratory practice.