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Agarose native gel electrophoresis for characterization of antibodies

Cynthia Li1, Teruo Akuta2, Masataka Nakagawa2

  • 1Alliance Protein Laboratories, a Division of KBI Biopharma, 6042 Cornerstone Court West, San Diego, CA 92121, United States of America.

International Journal of Biological Macromolecules
|February 22, 2020
PubMed

This study was conducted to evaluate applicability of the previously reported native agarose gel electrophoresis to the analysis of various monoclonal and polyclonal antibodies. Experiments were carried to test the electrophoresis system for characterization of different monoclonal antibodies and animal serum, analysis of expressed antibodies in cell culture and evaluation of antibody stability. An attempt to optimize the electrophoretic condition was made by adjusting the electrode buffer concentration, electrophoretic run time and agarose concentration.

Keywords:
AgaroseBasic proteinExpressionHistidineMonoclonal antibodyNative electrophoresis

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