Related Experiment Video

Updated: Aug 13, 2026

Assessment of Kidney Function in Mouse Models of Glomerular Disease
09:16

Assessment of Kidney Function in Mouse Models of Glomerular Disease

Published on: June 30, 2018

Heterogeneity of glomerular barrier function in early adriamycin nephrosis of MWF rats

M Soose1, U Haberstroh, G Rovira-Halbach

  • 1Department of Internal Medicine, Hannover Medical School, FRG.

Clinical Physiology and Biochemistry
|January 1, 1988
PubMed

Insights

Adriamycin (ADR) causes increased protein excretion in rats, indicating kidney damage. However, direct measurement in glomeruli suggests cortical nephrons are less affected than juxtamedullary ones.

Area of Science:

  • Nephrology
  • Toxicology
  • Molecular Biology

Background:

  • Adriamycin (ADR) is known to be toxic to glomerular epithelial cells.
  • Understanding the early effects of ADR on glomerular barrier function is crucial for kidney disease research.

Purpose of the Study:

  • To investigate the early impact of Adriamycin (ADR) on glomerular barrier function in MWF/Ztm rats.
  • To analyze changes in the excretion and filtration of albumin and high molecular weight (HMW) proteins following ADR administration.

Main Methods:

  • Administration of Adriamycin (5 mg/kg BW, i.v.) to MWF/Ztm rats.
  • Measurement of urinary protein excretion and glomerular filtration rate (GFR).
  • Free-flow micropuncture studies of superficial glomeruli to assess protein concentrations in Bowman's capsular space.

Main Results:

  • ADR administration led to a significant increase in urinary protein excretion after 7 days, with unchanged GFR.
  • Proteinuria was nonselective, affecting both albumin and HMW proteins without altering their ratio.
  • Micropuncture studies did not confirm increased protein concentration in Bowman's space, suggesting localized effects.

Conclusions:

  • ADR-induced proteinuria in final urine does not directly reflect glomerular filtration changes in superficial nephrons.
  • Cortical nephrons appear to be less sensitive to the toxic effects of ADR compared to juxtamedullary nephrons.
  • ADR's selective toxicity may involve differential sensitivity among nephron populations.

More Related Videos

An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney10:14

An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney

Published on: November 1, 2018

Highly Sensitive Measurement of Glomerular Permeability in Mice with Fluorescein Isothiocyanate-polysucrose 7009:16

Highly Sensitive Measurement of Glomerular Permeability in Mice with Fluorescein Isothiocyanate-polysucrose 70

Published on: August 9, 2019

Related Concept Videos

Renal Corpuscle01:20

Renal Corpuscle

The glomerulus and Bowman's capsule are two essential components of the nephron, which is the functional unit of the kidney. These microscopic structures play a critical role in the process of blood filtration to produce urine.
Glomerulus: Structure and Function
The glomerulus is a tiny, intricate network of capillaries located at the beginning of the nephron. It's enveloped by the Bowman's capsule and receives its blood supply from an afferent arteriole, which divides into numerous capillaries...
Glomerular Filtration Rate and its Regulation01:28

Glomerular Filtration Rate and its Regulation

The Glomerular Filtration Rate (GFR) is a measure of kidney function, reflecting the volume of filtrate formed per minute in the kidneys. On average, GFR is approximately 125 mL/min in males and 105 mL/min in females. Maintaining a relatively constant GFR is essential for the kidneys to effectively regulate body fluid homeostasis and maintain extracellular stability.
GFR regulation involves two primary intrinsic controls: the myogenic and tubuloglomerular feedback mechanisms.
The myogenic...
Renal Drug Excretion: Glomerular Filtration01:02

Renal Drug Excretion: Glomerular Filtration

The kidney serves as the primary organ responsible for eliminating drugs and their metabolites from the body. This process, known as renal elimination, starts with glomerular filtration and results in urine formation. Each kidney houses millions of functional units called nephrons, where urine production occurs. A nephron has two main components: a renal corpuscle and a renal tubule.
Drugs gain access to the kidney via the renal artery, which progressively branches off into afferent arterioles.
Factors Affecting Renal Clearance: Renal Impairment01:17

Factors Affecting Renal Clearance: Renal Impairment

Renal dysfunction significantly impairs the renal clearance of drugs, leading to potential complications in drug therapy. Renal failure, which can be caused by various factors, poses a significant challenge in the elimination of drugs from the body.
One condition associated with renal failure is uremia. Uremia is characterized by impaired glomerular filtration and fluid accumulation in the body. This condition hinders the renal clearance of drugs, resulting in drug accumulation and potential...
Drug Dosing in Renal Diseases: Measurement of Glomerular Filtration Rate01:25

Drug Dosing in Renal Diseases: Measurement of Glomerular Filtration Rate

The glomerular filtration rate (GFR) is a critical indicator of kidney health, reflecting how well the kidneys filter blood. Changes in GFR can signal potential kidney impairment, necessitating accurate measurement methods to monitor kidney function effectively.Various molecules can serve as markers for GFR measurement, with the ideal marker meeting several specific criteria. It must freely filter at the glomerulus, avoid reabsorption or secretion by the renal tubules, remain unmetabolized, not...