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Three-Dimensional Multilayered Microstructure Using Needle Array Bioprinting System.

Yasuhiro Shudo1, John W MacArthur1, Yoshihiro Kunitomi2

  • 1Department of Cardiothoracic Surgery, Stanford University School of Medicine, Stanford, California.

Tissue Engineering. Part A
|February 23, 2020
PubMed
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This summary is machine-generated.

This study introduces a robotic system for scaffold-free 3D tissue engineering, creating reproducible, graftable microstructures rapidly. The method promotes extracellular matrix formation, offering a promising strategy for regenerative therapies.

Area of Science:

  • Regenerative Medicine
  • Biotechnology
  • Materials Science

Background:

  • Tissue engineering is crucial for regenerative therapies.
  • Current methods face challenges in creating complex, functional tissues.
  • Scaffold-free approaches offer advantages by utilizing inherent cellular properties.

Purpose of the Study:

  • To introduce and evaluate a novel robotic system for scaffold-free 3D tissue fabrication.
  • To demonstrate the creation of multilayered microstructures using cell aggregation.
  • To assess the properties and potential applications of the engineered tissues.

Main Methods:

  • Utilized the "3D tissue fabrication system" (Regenova®) for automated cell culture and aggregation.
  • Cultured cells in non-adhesive wells to promote spheroid formation and extracellular matrix (ECM) deposition.
Keywords:
biofabricationextracellular matrixscaffold freethree-dimensionaltissue engineering

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  • Employed a computer-controlled robotic system for precise control over structure design and reproducibility.

    • Main Results:

    • Successfully created scaffold-free, three-dimensional (3D) multilayered microstructures from cultured cells.
    • Demonstrated designable, reproducible structures with controlled dimensions (length, size, shape).
    • Engineered tissues exhibited satisfactory mechanical properties and abundant ECM within 8 days, free from foreign materials.

    Conclusions:

    • The robotic 3D tissue fabrication system provides a practical and efficient method for generating graftable tissue constructs.
    • This scaffold-free approach promotes ECM formation and avoids issues associated with foreign materials.
    • The technology presents a promising strategy for advancing tissue engineering and regenerative medicine.