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Decreased shedding dipeptidyl peptidase 4 from membrane in Hashimoto's thyroiditis

Wenjie Xu1, Yongping Liu1, Xuebing Cheng1

  • 1Department of Endocrinology, Affiliated Hospital of Weifang Medical University, Weifang, Shandong 261031, China.

International Immunopharmacology
|February 23, 2020
PubMed
Abstract

Insights

Serum dipeptidyl peptidase 4 (DPP4) levels and activity are lower in Hashimoto

Area of Science:

  • Endocrinology
  • Immunology
  • Biochemistry

Background:

  • Hashimoto's thyroiditis (HT) is an autoimmune disease affecting the thyroid gland.
  • Dipeptidyl peptidase 4 (DPP4) is an enzyme with known roles in immune regulation.
  • Understanding DPP4's role in HT may reveal new therapeutic targets.

Purpose of the Study:

  • To quantify DPP4 concentration and enzymatic activity in serum and peripheral blood mononuclear cells (PBMCs) of HT patients.
  • To investigate the relationship between DPP4 and other factors in HT pathogenesis.
  • To explore the potential mechanism behind altered DPP4 in HT.

Main Methods:

  • Enrolled 33 newly diagnosed HT patients and 31 healthy controls.
  • Measured serum DPP4 and kallikrein-related peptidase 5 (KLK5) using ELISA.
  • Assessed DPP4 enzymatic activity and mRNA expression in serum and PBMCs.
  • Evaluated KLK5 and interferon-gamma (IFN-γ) mRNA expression in PBMCs via qPCR.

Main Results:

  • HT patients showed significantly lower serum DPP4 levels and activity compared to controls.
  • DPP4 mRNA expression was elevated in HT patients' PBMCs.
  • Serum KLK5 concentration and KLK5 mRNA expression in PBMCs were significantly reduced in HT patients.
  • IFN-γ mRNA expression in PBMCs was significantly increased in HT patients.

Conclusions:

  • DPP4 plays a role in the development and pathology of Hashimoto's thyroiditis.
  • Reduced cleavage of membrane-bound DPP4 by KLK5 may contribute to lower serum DPP4 levels in HT.
  • Altered DPP4 and KLK5 levels suggest a complex interplay in HT pathogenesis.
Keywords:
Autoimmune thyroid diseaseDipeptidyl peptidase 4Hashimoto's thyroiditisKallikrein-related peptidase 5

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