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Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Dec 28, 2025

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Published on: July 10, 2016

Continuous Cell Separation Using Microfluidic-Based Cell Retention Device with Alternative Boosted Flow.

Po-Hung Chen¹, Yu-Ting Cheng¹, Bing-Syuan Ni¹

¹Department of Chemical Engineering, National Tsing Hua University, No. 101, Sec. 2, Kuang-Fu Rd., Hsinchu, 30013, Taiwan.

Applied Biochemistry and Biotechnology

|February 23, 2020

Summary

This summary is machine-generated.

A novel microfluidic device enables continuous cell separation, reducing costs and facility size for bioprocessing. This innovative system achieves high cell viability and concentration, proving promising for downstream applications.

Keywords:

Antifouling Cell separation Continuous bioprocess Cross-flow filtration Microfluidics

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Last Updated: Dec 28, 2025

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

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A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

Area of Science:

Biotechnology
Chemical Engineering
Microfluidics

Background:

Continuous manufacturing is increasingly important for cost-effective bioprocessing.
Conventional batch cell separation methods are costly and require large facilities.
There is a need for efficient and scalable continuous cell separation technologies.

Purpose of the Study:

To develop a microfluidic device for continuous, size-based cell separation.
To overcome membrane fouling issues in continuous filtration systems.
To demonstrate the efficacy of the device for downstream bioprocessing.

Main Methods:

Fabrication of a multi-layered microfluidic device with porous membranes.
Implementation of restrictive cross-flow filtration for size-based cell isolation.
Utilizing an alternative flow rate strategy to mitigate membrane fouling.

Main Results:

The device successfully separated cells into two streams: one with cells and one with conditioned medium.
An alternative boosted flow strategy achieved a high permeate flow rate of 0.3 mL/min.
The process maintained high cell viability (>98%) and increased cell concentration by 48%.

Conclusions:

The microfluidic-based continuous cell separation system is a viable alternative to batch processes.
This technology offers reduced capital costs and facility footprint for biomanufacturing.
The system shows significant potential for improving downstream bioprocessing efficiency.