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Related Experiment Video

Updated: Dec 28, 2025

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

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Establishment and characterization of silver-resistant Enterococcus faecalis.

Jingwen Cui¹, Qing Sun¹, Mengting Duan¹

¹The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan, 430079, People's Republic of China.

Folia Microbiologica

|February 23, 2020

Summary

This summary is machine-generated.

Enterococcus faecalis develops resistance to silver ions (Ag+) and nanoparticles (AgNPs). Gene Ontology analysis revealed significant differences in gene expression, providing insights into managing silver resistance in infections.

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Area of Science:

Microbiology
Bacterial Resistance
Nanomaterials in Medicine

Background:

Enterococcus faecalis is a Gram-positive bacterium implicated in severe human infections.
Silver ions (Ag+) and nanoparticles (AgNPs) are broad-spectrum antimicrobials, yet E. faecalis exhibits resistance.
Understanding E. faecalis silver resistance is crucial for effective infection control.

Purpose of the Study:

To establish and characterize silver-resistant Enterococcus faecalis strains.
To compare the biological and silver-resistant properties of these strains with the original E. faecalis.
To investigate gene expression differences using Gene Ontology (GO) analysis.

Main Methods:

Serial selection method to generate silver-resistant E. faecalis (AgR and ANR strains).
Determination of minimum bactericidal concentrations (MBCs) for AgNO3 and AgNPs.
Gene Ontology (GO) analysis to compare gene expression profiles.

Main Results:

Silver-resistant E. faecalis strains (AgR, ANR) maintained proliferative capacity and showed enhanced resistance to both Ag+ and AgNPs.
MBCs for AgNO3 were 400 mg/L (original), 600 mg/L (AgR), and 500 mg/L (ANR).
MBCs for AgNPs were 80 mg/L (original), 110 mg/L (AgR), and 130 mg/L (ANR).
Significant differences (P < 0.05) in gene expression related to biological process, cellular component, and molecular function were observed via GO analysis.

Conclusions:

Established silver-resistant E. faecalis strains exhibit increased resistance to silver ions and nanoparticles.
Gene expression profiles differ significantly between original and silver-resistant strains.
Findings provide a basis for understanding and managing silver resistance in E. faecalis infections, though resistance mechanisms require further investigation.